Addgene

Showing: 1621 - 1640 of 2899 results

Results per page:

Sort results by:

pDS277

Plasmid

#34973

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

V529N

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Syn-PSD95.FingR-eGFP

Plasmid

#126212

Purpose

Labeling of excitatory synapses (green fluorescence) in dividing cells

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Use

Tags

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV Syn intron Psam4GlyR m3m4 HA

Plasmid

#196041

Purpose

AAV for Chemogenetic plasmids

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

Psam4GlyR m3m4 HA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LZF39: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104118

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Plasmid

#214265

Purpose

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

Depositor

D. James Surmeier

Insert

Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

Synapsin

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Expression

Mutation

F102G

Promoter

Synapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

Plasmid

#104116

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-Citrine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-CRY2Clust

Plasmid

#200969

Purpose

induction of phase-separated condensate by CRY2Clust

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

CRY2Clust

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAurora

Plasmid

#190158

Purpose

Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCrepusculo

Plasmid

#190156

Purpose

Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-WP1-CRY2PHR-VP64

Plasmid

#171758

Purpose

Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

WP1-CRY2PHR-VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Synthetic, Mustard Weed)

Use

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NBid-PhoCl1-CBid

Plasmid

#164050

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl1

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl1-CBid

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSW FLEx mCherry-KASH

Plasmid

#139652

Purpose

Conditional expression of mCherry-KASH

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry-KASH

Use

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge