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We narrowed to 2,253 results for: 3-Oct

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Showing: 1621 - 1640 of 2253 results

  1. pBBR1-EcTrpRS(H14)

    Plasmid
    #231131
    Purpose
    Expresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tryptophanyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    S8A, V144G, V146C
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231131-plasmid-map-sequence-id-458351

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  2. pBBR1-EcTyrRS(VSMA,D165G)

    Plasmid
    #231132
    Purpose
    Expresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tyrosyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    Y37V, D165G, D182S, P183M, L186A, D265R
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231132-plasmid-map-sequence-id-458349

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  9. NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)

    Plasmid
    #191165
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTR
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587-3xMS2:EGFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191165-plasmid-map-sequence-id-375483

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  15. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  16. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  17. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid
    #216164
    Purpose
    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Gaussia princeps luciferase (with double mutation to improve stability)
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216164-plasmid-map-sequence-id-437759

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  18. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid
    #216165
    Purpose
    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mCherry with C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216165-plasmid-map-sequence-id-437747

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  19. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

    Plasmid
    #216162
    Purpose
    Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216162-plasmid-map-sequence-id-437755

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  20. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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Showing: 1621 - 1640 of 2253 results