-
Plasmid#120476PurposeBarcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSIX1 (SIX1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #2
Plasmid#83091PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4DepositorInsertshSMAD4 (SMAD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-WP1-CRY2PHR-VP64
Plasmid#171758PurposeLentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64DepositorInsertWP1-CRY2PHR-VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRecLV103-GFP-PGBD5
Plasmid#65409PurposeLentiviral expression vector for fusion of GFP with human PGBD5DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROG1_P2A_Hygro_Barcode
Plasmid#120467PurposeBarcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROG1 (NEUROG1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-Gateway
Plasmid#114011Purpose3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Notch1 del-NRR
Plasmid#188353PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgUFSP2
Plasmid#86134PurposeLentiviral vector expressing Cas9 and an sgRNA targeting UFSP2DepositorInsertsgRNA targeting UFSP2 (UFSP2 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinVenus-S1033A
Plasmid#211896PurposeVinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.DepositorInsertVinculinVenus-S1033A (VCL Chicken)
UseLentiviralTagsVenusA206KExpressionMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinVenus-S1033D
Plasmid#211897PurposeVinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.DepositorInsertVinculinVenus-S1033D (VCL Chicken)
UseLentiviralTagsVenusA206KExpressionMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-4493NES
Plasmid#175811PurposeThe lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.DepositorInsertBooster-PKA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo empty (w36-1)
Plasmid#17478Purpose3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, NeoDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-MTS-FLAG-LOV*
Plasmid#202208PurposePlasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cellsDepositorInsertCytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
UseLentiviralTagsFLAG and LOV*ExpressionMutationPromoterEf1aAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMPC2_7
Plasmid#163457Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 7 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMPC2_9
Plasmid#163458Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 9 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-CSE1L zeo
Plasmid#192320PurposeLentiviral expression vector for an inducible 3xFLAG-CSE1LDepositorInsertCSE1L (CSE1L Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP
Plasmid#192609PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.DepositorInsertLexA-CIB1-biLINuS-P2A-tagBFP
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only