We narrowed to 12,966 results for: lic

1. pET32-MCM2/7

   Plasmid

   #116949

   Purpose

   Expresses human MCM2 and human MCM7

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM7 (MCM7 Human)
   Human MCM2 (MCM2 Human)
   

   Tags

   Thioredoxin, His6

   Expression

   Bacterial

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-RAD52-∆C

   Plasmid

   #235677

   Purpose

   Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 302-410

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-mCherry-2A-BRD4 Iso AdelCTD

   Plasmid

   #137722

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated at amino acid 1328

   Promoter

   Tight TRE promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-mCherry-2A-BRD4 Iso CdelET

   Plasmid

   #137723

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted amino acids 600-682

   Promoter

   Tight TRE promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPC-NMYC-hTRF1deltaBLM

   Plasmid

   #64165

   Purpose

   Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deleted amino acids aa317-374 of human TRF1

   Promoter

   CMV

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-SEPT_Cdk2-T160E-siR

   Plasmid

   #73976

   Purpose

   HDR template to generate Cdk2-T160E-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160E (CDK2 Human)
   

   Use

   AAV

   Mutation

   T160 mutated to Glu and siRNA target site mutated

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX209-neo-WRN K577M

   Plasmid

   #125790

   Purpose

   constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN K577M (WRN Human)
   

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   K577M (helicase-dead)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX209-neo-WRN E84A K577M

    Plasmid

    #125791

    Purpose

    constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN E84A/K577M (WRN Human)
    

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M (exonuclease/helicase-dead)

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZL Neo Myr Flag CAMK1G

    Plasmid

    #20438

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CAMK1G (CAMK1G Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. OA-1136J

    Plasmid

    #153023

    Purpose

    Express His-MBP-TEV-CasRx recombinant protein

    Depositor

    Omar Akbari

    Article

    Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

    Insert

    CasRx
    

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only