We narrowed to 168,660 results for: addgene

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MAC_N_ARPC1B

Plasmid

#111681

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARPC1B (ARPC1B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-LAMP1

Plasmid

#207787

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_WT

Plasmid

#181920

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

Depositor

Christian Metallo

Article

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154787

Purpose

Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Synthetic, Fly)

Use

Lentiviral

Tags

GFP, V5, and stdMCP

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pWPI-GFP-3xFLAG

Plasmid

#201639

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_Zeo

Plasmid

#118596

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2

Plasmid

#228864

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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