We narrowed to 13,284 results for: sequence
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Plasmid#133332PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
Tags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]
Plasmid#118813PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationG368W and W385G in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
Plasmid#118808PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll F to S in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]
Plasmid#118809PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]
Plasmid#118810PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds
Plasmid#119070PurposeConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled1-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds
Plasmid#119071PurposeConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds
Plasmid#119072PurposeConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled3-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only