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Showing: 2461 - 2490 of 13739 results

  1. CD84-bio-His

    Plasmid
    #51649
    Purpose
    Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD84 (CD84 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51649-plasmid-map-sequence-id-78973

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  3. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid
    #122500
    Purpose
    Cre-dependent GFP expression.
    Depositor
    Kimberly Ritola
    Insert
    3x GFP
    Use
    AAV
    Mutation
    each GFP has unique nt sequence
    Available Since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    122500-plasmid-map-sequence-id-237802

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  4. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    HindIII
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

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  5. pSARGENT1

    Plasmid
    #216315
    Purpose
    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence
    Depositor
    Barak Cohen
    Insert
    tagBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216315-plasmid-map-sequence-id-430346

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  6. pGEX-CS-MjPiwi_G

    Plasmid
    #146384
    Purpose
    Bacterial Expression of MjPiwi
    Depositor
    Elisa Izaurralde
    Insert
    MjPiwi
    Expression
    Bacterial
    Mutation
    one missense mutation C490A (H163N) compared to t…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146384-plasmid-map-sequence-id-288829

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  7. pcDNA3.1-HA-Bax-mito

    Plasmid
    #191943
    Purpose
    Expression of Bax and mitochodrial targeting sequence.
    Depositor
    Luca Scorrano
    Insert
    Bax-mito
    Expression
    Mammalian
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    191943-plasmid-map-sequence-id-379566

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  8. pUPD2 GFP (GB2237)

    Plasmid
    #160591
    Purpose
    GFP coding sequence.
    Depositor
    Diego Orzaez
    Insert
    GFP
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160591-plasmid-map-sequence-id-322075

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  9. pGGD_RT_biChromium

    Plasmid
    #136974
    Purpose
    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide
    Depositor
    Klaus Theres
    Insert
    P2A sequence
    Expression
    Bacterial
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136974-plasmid-map-sequence-id-270595

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  10. pQE-60 roGFP2-His

    Plasmid
    #65046
    Purpose
    Bacterial expression of His-tagged roGFP2
    Depositor
    Tobias Dick
    Insert
    roGFP2
    Tags
    His
    Expression
    Bacterial
    Mutation
    see sequence
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65046-plasmid-map-sequence-id-350692

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  11. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRI
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  12. pOpen-EcoRV

    Plasmid
    #165548
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRV
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165548-plasmid-map-sequence-id-339428

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  13. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst HF
    Use
    Synthetic Biology
    Available Since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

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  14. pOpen-SapIR

    Plasmid
    #165537
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SapI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165537-plasmid-map-sequence-id-339127

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  15. pMSCV2.2-IRES- GFP-Opa1

    Plasmid
    #191971
    Purpose
    Plasmid for cloning reaction, containing OPA1 coding sequence.
    Depositor
    Luca Scorrano
    Insert
    GFP-Opa1
    Use
    Retroviral
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191971-plasmid-map-sequence-id-379642

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  16. hU6-Sa_tracr

    Plasmid
    #135973
    Purpose
    Contains tracrRNA-hU6 sequence for double sgRNA cloning
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135973-plasmid-map-sequence-id-313948

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  17. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.PstI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  18. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    XbaI
    Use
    Synthetic Biology
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165567-plasmid-map-sequence-id-340099

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  19. pOpen-NcoIR

    Plasmid
    #165568
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NcoI
    Use
    Synthetic Biology
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165568-plasmid-map-sequence-id-340100

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  20. pDIV1049

    Plasmid
    #247970
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target mCitrine. pyrG marker
    Depositor
    Uffe Mortensen
    Insert
    Cas9-AMA1-mCit-gRNA-pyrG-Ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available Since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    247970-plasmid-map-sequence-id-503025

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  21. pCAGGS-MTS-EGFP-V5

    Plasmid
    #200816
    Purpose
    expresses MTS-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200816-plasmid-map-sequence-id-409411

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  22. pETM-11-DmPABPC1_501-634_J

    Plasmid
    #146641
    Purpose
    Bacterial Expression of HsPABPC1_501-634
    Depositor
    Elisa Izaurralde
    Insert
    HsPABPC1_501-634
    Expression
    Bacterial
    Mutation
    2 silent mutations compared to the sequence given…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146641-plasmid-map-sequence-id-289087

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  23. pAc5.1B-lambdaN-HA-HsTNRC6C_G

    Plasmid
    #146374
    Purpose
    Insect Expression of HsTRC6C
    Depositor
    Elisa Izaurralde
    Insert
    HsTRC6C
    Expression
    Insect
    Mutation
    two silent mutations C2349T and A2547C compared t…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146374-plasmid-map-sequence-id-288819

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  24. pDEST-Opa1

    Plasmid
    #191961
    Purpose
    Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology
    Depositor
    Luca Scorrano
    Insert
    Opa1
    Use
    Synthetic Biology
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191961-plasmid-map-sequence-id-379662

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  25. pOME0276M

    Plasmid
    #182614
    Purpose
    Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275
    Depositor
    Dirk Dittmer
    Insert
    Reverse compliment HHV8 TO_7 control
    Expression
    Mammalian
    Available Since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182614-plasmid-map-sequence-id-359450

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  26. pOME0237M

    Plasmid
    #182599
    Purpose
    Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Dirk Dittmer
    Insert
    Reverse compliment of anti-PAN (pOME0236)
    Expression
    Mammalian
    Available Since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182599-plasmid-map-sequence-id-359434

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  27. PTPRC-bio-His

    Plasmid
    #51813
    Purpose
    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRC (PTPRC Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51813-plasmid-map-sequence-id-79539

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  28. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  29. LRP8-bio-His

    Plasmid
    #51879
    Purpose
    Expresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRP8 (LRP8 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51879-plasmid-map-sequence-id-79841

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  30. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid
    #198240
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198240-plasmid-map-sequence-id-393627

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Showing: 2461 - 2490 of 13739 results