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pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)
Plasmid#70194PurposeDerived from 21 with silent mutations to make BsaI, BbsI compatibleDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
UseTags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE76
Plasmid#80578Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptIIDepositorInsertspnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-PhCHS-CmCHI
Plasmid#73397PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9noSsrA
Plasmid#102285PurposeModified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationremoved ssrA tagPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE77
Plasmid#80579Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTADepositorInsertspnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE78
Plasmid#80580Purposerecipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptIIDepositorInsertspnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE79
Plasmid#80581Purposerecipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTADepositorInsertspnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1032
Plasmid#52997PurposepLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pYihI-C-term K-R
Plasmid#233092PurposeExpression of GST-YihI with C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with C-term lysines (K) mutated to arginine (R)
UseTagsGSTExpressionBacterialMutationC-term lysines (K) mutated to arginine (R)PromoterAvailable sinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
UseTags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
SBC015898
Plasmid#226285PurposeExpresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.DepositorInsertsConiferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Tir
Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR88
Plasmid#203422Purposeempty BLINCAR plasmid for payload delivery, no payload, no genomic targetDepositorInsertsCAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PGUS
Plasmid#207827PurposeFLAG-tagged PGUS driven by CMV promoterDepositorInsertPGUS
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyTagsExpressionMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-GUS
Plasmid#202137PurposeLevel 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.DepositorInsertBeta glucuronidase gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterP-LlacO-1Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyTagsExpressionMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVioCD53
Plasmid#191130PurposeExpression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoterDepositorInsertsUseSynthetic BiologyTagsExpressionMutationC-terminal truncation from 264th amino acidPromoterJ23105 constitutive promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-P19-NP
Plasmid#196608PurposesiRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeatDepositorInsertp19 + Influenza virus NP inverted repeat
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- BAD-YFP
Plasmid#186567PurposepMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-HpaB-9
Plasmid#197756PurposePhytobrick (MoClo) Level 0 PartDepositorInsertFBP: HpaB (caffeic acid biosynthesis)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-HpaC-9
Plasmid#197757PurposePhytobrick (MoClo) Level 0 PartDepositorInsertFBP: HpaC (caffeic acid biosynthesis)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1954
Plasmid#195689PurposeExpression/purification of 6xHis-TEV-MlaD(F118A/L123A)DepositorInsertMlaD
UseTagsExpressionBacterialMutation6xHis-TEV-MlaD(F118A/L123A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1936
Plasmid#195692PurposeComplementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaDdelta153-183-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1935
Plasmid#195693PurposeComplementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaDdelta173-183-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1953
Plasmid#195694PurposeComplementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1933
Plasmid#195695PurposeComplementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaD(F118A)-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1934
Plasmid#195696PurposeComplementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaD(L123A)-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
UseTagsExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2707
Plasmid#195636PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2753
Plasmid#195637PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; I241N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2754
Plasmid#195638PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; L245N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2755
Plasmid#195639PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; I248N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2756
Plasmid#195640PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2757
Plasmid#195641PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2766
Plasmid#196028PurposeComplementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-MlaE-MlaDdelta141-183-MlaC-MlaBPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-NP
Plasmid#196607PurposeDual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward directionDepositorInsertnucleocapsid protein (NP) gene, partial
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-T7- YFP
Plasmid#186568Purposep15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only