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Showing: 4101 - 4150 of 9181 results
  1. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pMiNGo11-1

    Plasmid
    #126056
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pHL 1032

    Plasmid
    #52997
    Purpose
    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pYihI-C-term K-R

    Plasmid
    #233092
    Purpose
    Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)
    Depositor
    Insert
    GST-YihI with C-term lysines (K) mutated to arginine (R)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-term lysines (K) mutated to arginine (R)
    Promoter
    Available since
    May 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. SBC015898

    Plasmid
    #226285
    Purpose
    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.
    Depositor
    Inserts
    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    Promoter
    P-LlacO-1
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pTP-PCR C1

    Plasmid
    #189618
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pVioCD53

    Plasmid
    #191130
    Purpose
    Expression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoter
    Depositor
    Inserts
    vioC (vioC Synthetic)
    vioD truncated
    dMCP off-frame
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C-terminal truncation from 264th amino acid
    Promoter
    J23105 constitutive promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pTP-PCR C4

    Plasmid
    #189621
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, and minor additional mutat…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pTP-PCR C2

    Plasmid
    #189619
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 4101 - 4150 of 9181 results