We narrowed to 1,747 results for: lin tian

1. pCB59 pDEST crybb1:mKate2

   Plasmid

   #195986

   Purpose

   Transgenesis backbone

   Depositor

   Markus Affolter, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM246 - MCS mosTI unc-119 target

   Plasmid

   #159821

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI unc-119 target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Myc-ASC

   Plasmid

   #73952

   Purpose

   Express c-Myc-tagged human ASC in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-21a(+)-AK1-His

   Plasmid

   #118977

   Purpose

   Bacterial expression plasmid for Adenylate kinase isoenzyme 1

   Depositor

   Sebastian Maerkl

   Article

   Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

   Insert

   adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-pro-IL1b

   Plasmid

   #73953

   Purpose

   Express murine pro-interleukin-1 beta in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

   Insert

   interleukin-1 beta (Il1b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

   Plasmid

   #182353

   Purpose

   GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEM320 - sgRNA mosTI unc-119 array insertion

   Plasmid

   #159824

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 array insertion
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

   Plasmid

   #182344

   Purpose

   sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Spacer 4
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCK082 desmaMCS:minprommCherry,exorh:mCerulean

    Plasmid

    #200015

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Leonard Zon, Christian Mosimann

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    ubi

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM272 - MCS mosTI Pmlc-2_GFP target

    Plasmid

    #159834

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI Pmlc-2_GFP target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

    Plasmid

    #182352

    Purpose

    Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM255 - sgRNA mosTI Pmlc-2_GFP

    Plasmid

    #159835

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI Pmlc-2_GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

    Plasmid

    #182348

    Purpose

    mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-DHFR

    Plasmid

    #80329

    Purpose

    Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    DHFR-TSc3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    DHFR-TS 5' UTR

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-SAG1-DHFR

    Plasmid

    #80322

    Purpose

    Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    SAG1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

    Plasmid

    #200016

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Use

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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