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pPOTv6-g418-3Ty:mNG:3Ty

Plasmid

#179800

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR hH1-ARRET

Plasmid

#111382

Purpose

ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

Depositor

Klaus Holzmann

Insert

hH1-ARRET

Use

Gateway entry cloning

Promoter

hH1

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW211J109-Amp32C

Plasmid

#78646

Purpose

pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pME-BTK-PH

Plasmid

#67673

Purpose

Gateway entry clone containing BTK-PH with no stop codon

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

BTK-PH-NS

Use

Gateway multisite middle entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PEST2D

Plasmid

#62434

Purpose

MXS_chaining vector with PEST2D

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Destabilization tag

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Publication

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
T2A-mCherry (MTK 4a) (pAN3844)

Plasmid

#194223

Purpose

Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

Depositor

Hana El-Samad

Publication

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

T2A-mCherry

Use

Synthetic Biology

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NK612

Plasmid

#109092

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Publication

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact-mCherry

Use

Choanoflagellate expression

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Publication

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Promoter

T7 (2X)

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR R4-GAL4-R3

Plasmid

#32309

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

GAL4 (GAL4 Budding Yeast)

Use

Gateway multisite entry clone

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pME-Zebrabow

Plasmid

#226792

Purpose

Middle-entry vector with Zebrabow cassette. For use in Gateway cloning.

Depositor

Florence Marlow

Publication

Pahima et al Development. 2026 Jul 6:dev.204904. doi:

Insert

Zebrabow

Use

Gateway cloning middle-entry vector

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3HA::FRB::3HA

Plasmid

#247588

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBK-EcTrpRS(H14)

Plasmid

#231129

Purpose

Expresses mutant E. coli TrpRS from a weak glnS promoter for proteome-wide incorporation of tryptophan analogues.

Depositor

Abhishek Chatterjee

Publication

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tryptophanyl tRNA synthetase

Expression

Bacterial

Mutation

S8A, V144G, V146C

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pR-mT-Sapphire

Plasmid

#228597

Purpose

Mammalian expression of mT-Sapphire, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mT-Sapphire

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mCerulean3

Plasmid

#228599

Purpose

Mammalian expression of mCerulean3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mPapaya

Plasmid

#228607

Purpose

Mammalian expression of mPapaya, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mPapaya

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Publication

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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DT043A

Plasmid

#159404

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

T7 bacteriophage promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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