164,577 results

1. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-HA-BHLHE40

   Plasmid

   #110154

   Purpose

   Expression of human BHLHE40 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDESTmycDICER

   Plasmid

   #19873

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   dicer 1, ribonuclease type III (DICER1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lv EF1a dCas9-HA 2A Blast

   Plasmid

   #102812

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   dCas9-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A (dCas9)

   Promoter

   EF1a

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tom20-CIB-GFP

   Plasmid

   #117242

   Purpose

   Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   CIBN
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMK346 (Hygro-P2A-mAID-mCherry)

   Plasmid

   #121180

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. plRL117

   Plasmid

   #163635

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-APP-mGFP

   Plasmid

   #196704

   Purpose

   Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mCherry and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge