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Showing: 2491 - 2520 of 8793 results

  8. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  11. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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  12. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid
    #114078
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    Use
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114078-plasmid-map-sequence-id-220564

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  15. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180152
    Purpose
    tdTomato cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180152-plasmid-map-sequence-id-355317

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  19. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid
    #168106
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning
    Depositor
    Erich Jarvis
    Article
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Insert
    mCherry-KASH
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168106-plasmid-map-sequence-id-330309

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  21. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68717
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    CAG-FLEX
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68717-plasmid-map-sequence-id-120856

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  23. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180156
    Purpose
    tdTomato cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180156-plasmid-map-sequence-id-360161

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  26. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180153
    Purpose
    td-sfGFP dre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180153-plasmid-map-sequence-id-355318

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  29. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid
    #172479
    Purpose
    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172479-plasmid-map-sequence-id-338913

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Showing: 2491 - 2520 of 8793 results