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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,028 results for: pET

Showing: 2491 - 2520 of 15028 results

  2. pET29a-DDX53-Q249A

    Plasmid
    #222216
    Purpose
    Express DDX53 mutant Q249A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_Q249A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222216-plasmid-map-sequence-id-441427

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  3. pET29a-DDX53-K272A

    Plasmid
    #222215
    Purpose
    Express DDX53 mutant K272A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_K272A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222215-plasmid-map-sequence-id-441662

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  4. pET29a-DDX53-E377Q

    Plasmid
    #222217
    Purpose
    Express DDX53 mutant E377Q protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_E377Q (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    E377Q
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222217-plasmid-map-sequence-id-441653

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  5. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

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  6. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

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  7. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

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  9. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  17. pET41-FeoB-OL4del

    Plasmid
    #216744
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Promoter
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216744-plasmid-map-sequence-id-434438

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  18. pET41-FeoB-OL5del

    Plasmid
    #216745
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 5 (OL5) with the sequence of [ATFAA] i…
    Promoter
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216745-plasmid-map-sequence-id-434437

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  19. pET41-FeoB-OL1del

    Plasmid
    #216741
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 1 (OL1) with the sequence of [INIGGALQ…
    Promoter
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216741-plasmid-map-sequence-id-434055

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  20. pET28-RPL3L-TwStrep

    Plasmid
    #208649
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949
    Depositor
    Quanfu Ma
    Insert
    human RPL3L (ENST00000268661) (RPL3L Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    contain rs753338989, rs746044959, rs752134949, pr…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208649-plasmid-map-sequence-id-433652

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  21. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

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Showing: 2491 - 2520 of 15028 results