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Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
UseTags6xHisExpressionYeastMutationG951A,C957APromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV114
Plasmid#63181PurposepRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-CPR
Plasmid#126742Purposeyeast plasmid overexpressing CPRDepositorInsertcpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ACS1
Plasmid#126712Purposeyeast plasmid complementing ACS1 from Komagataella phaffiiDepositorInsertacs1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dGZmxh
Plasmid#44552DepositorInsertsPTETREG promoter
yEGFP::ZeoR
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Pfba1-PYC-Trpl15a
Plasmid#127738PurposeYeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceJan. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ab-Psptef1-NAT-Ttip1
Plasmid#127747PurposeYeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.DepositorInsertnourseothricin N-acetyltransferase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptef1Available sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneUseTagsCmyc and HAExpressionYeastMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-eGFP
Plasmid#126716Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTags6xHisExpressionYeastMutationPromoterICL1Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Phhf2-PYC-Tadh1
Plasmid#127737PurposeYeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhhf2Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ACS1-eGFP
Plasmid#126715Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterACS1Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ALD-eGFP
Plasmid#126714Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterALDAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-HXT1-eGFP
Plasmid#126719Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterHXT1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ADH3-eGFP
Plasmid#126713Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterADH3Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ALD1
Plasmid#126711Purposeyeast plasmid complementing ALD1 from Komagataella phaffiiDepositorInsertald1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ADH3
Plasmid#126710Purposeyeast plasmid complementing ADH3 from Komagataella phaffiiDepositorInsertadh3
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-sAR
Plasmid#126743Purposeyeast plasmid overexpressing CPR and sLovADepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]
Plasmid#106462PurposeExpresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W(N163,242Q)
UseTagsPAExpressionBacterial and YeastMutationchanged Asparagines at positions 163 and 242 to G…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]
Plasmid#106466PurposeExpresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]
Plasmid#106467PurposeExpresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Histidine 474 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]
Plasmid#106468PurposeExpresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(D415A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Aspartate 415 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mECitrine
Plasmid#105152PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mECitrine
UseTags41nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter41nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only