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Plasmid#179109PurposeACTB N-term Nicking guide 1 +48 guideDepositorInsertACTB N-term Nicking guide 1 +48 guide
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcw57.1-RquA-FLAG
Plasmid#229685PurposeDoxycycline-inducible expression of RquA-FLAGDepositorInsertRquA-FLAG
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
DRH002_scAAV-hSyn-delta_iCre-HA
Plasmid#225087PurposeExpression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.DepositorInsertdelta_iCre
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterhSynAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY0216 PASTEv1 PE2-P2A-Bxb1
Plasmid#179104PurposePASTEv1 PE2-P2A-Bxb1DepositorInsertpCMV-PE2-P2A-Bxb1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0750 pCAG-NLS-BceINT
Plasmid#179112PurposeBceINT expression vectorDepositorInsertpCAG-NLS-BceINT
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
UseTagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG SARS-CoV2 RdRp.co
Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
UseTags3xFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Human, Synthetic)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0738 pCAG-NLS-SscINT
Plasmid#179113PurposeSscINT expression vectorDepositorInsertpCAG-NLS-SscINT
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0739 pCAG-NLS-SacINT
Plasmid#179114PurposeSacINT expression vectorDepositorInsertpCAG-NLS-SacINT
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-GSX2-IRES2-EBF1
Plasmid#96966PurposeDox-inducible expression of Gsx2 and Ebf1 in mammalian cellsDepositorUseTagsFLAG (on EBF1)ExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDY0310 TP901-1 Integrase
Plasmid#179110PurposeTP901-1 Integrase expression vectorDepositorInsertTP901-1 Integrase
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNK115
Plasmid#219745PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK116
Plasmid#219742PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGG-AH-VRT-A2
Plasmid#163703PurposeWheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)DepositorInsertsOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
UseTagsExpressionPlantMutationPromoterRice - Os Actin promoter and Triticum polonicum V…Available sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-6HIS-TEV-mEGFP,IRES-NGFR
Plasmid#164521PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-U6-sgRNA-BFP-Puro
Plasmid#229014PurposePerturb Seq lentiviral sgRNA vectorDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEGF120TAG
Plasmid#200946PurposeEncodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporationDepositorInsertsUseLentiviralTags6x HISExpressionMutationamber stop codon 101 bp from ATGPromoterEf1a and hPGKAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
VEGF164TAG
Plasmid#200947PurposeEncodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporationDepositorInsertsUseLentiviralTags6x HISExpressionMutationamber stop codon 101 bp from ATGPromoterEf1a and hPGKAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only