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Showing: 1661 - 1680 of 5470 results

  5. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  6. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

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  7. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

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  10. VEGF120TAG

    Plasmid
    #200946
    Purpose
    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Expression
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200946-plasmid-map-sequence-id-396320

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  11. VEGF164TAG

    Plasmid
    #200947
    Purpose
    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Expression
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200947-plasmid-map-sequence-id-396321

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  12. ρ1-EM GABAA receptor in pEZT-BM

    Plasmid
    #213072
    Purpose
    Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD
    Depositor
    Erik Lindahl
    Article
    Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.
    Insert
    Human GABAa rho1 receptor (GABRR1 Human)
    Use
    Bacmam, baculovirus, oocyte expression
    Tags
    Twin-Strep tag and superfolderGFP
    Expression
    Mammalian
    Mutation
    Encodes residues S58–L383 and D451–S479, separate…
    Promoter
    CMV and T7
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213072-plasmid-map-sequence-id-423917

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  14. pCDF-mcherry1

    Plasmid
    #87144
    Purpose
    Constitutive E. coli mcherry Expression
    Depositor
    Michael Lynch
    Insert
    mcherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive proD
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87144-plasmid-map-sequence-id-176832

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  15. pCDF-mcherry2

    Plasmid
    #87145
    Purpose
    Constitutive E. coli mcherry Expression, with DAS4 tag
    Depositor
    Michael Lynch
    Insert
    mcherry
    Use
    Tags
    C terminal DAS4 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive proD
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87145-plasmid-map-sequence-id-176838

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  16. pBT1-proD-mCherry

    Plasmid
    #65823
    Purpose
    Const. mCherry expression , proD promoter, E. coli Expression
    Depositor
    Michael Lynch
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    proD E . coli Constitutive Promoter
    Available since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65823-plasmid-map-sequence-id-111984

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  17. pICT19r

    Plasmid
    #1430
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    lacZN, SupTrp5', SupTrp3'
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1430-plasmid-map-sequence-id-41

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  18. pAST_19a

    Plasmid
    #1427
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    lacZN, SupTrp3', SupTrp5'
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1427-plasmid-map-sequence-id-38

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  19. pPD107.09

    Plasmid
    #1703
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1703-plasmid-map-sequence-id-309

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  20. pAST_18b

    Plasmid
    #1426
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    lacZN, SupTrp3', SupTrp5'
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1426-plasmid-map-sequence-id-37

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Showing: 1661 - 1680 of 5470 results