We narrowed to 5,521 results for: cycs
-
Plasmid#169577PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline repressor protein
ExpressionMammalianPromoterPhCMVAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-AUR1-msGFP2
Plasmid#177101PurposeExpresses Aur1-msGFP2 in yeast cellsDepositorInsertAUR1 (AUR1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RBD2-6xmGFP
Plasmid#177109PurposeExpresses Rbd2-6xmEGFP in yeast cellsDepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC2-msGFPx3
Plasmid#177110PurposeExpresses Sec2-msGFPx3 in yeast cellsDepositorInsertSEC2 (SEC2 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YSBE415: pTOP_ACA1_Vc_HOG1 terminator
Plasmid#122434PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE416: pTOP_CAC1_Vn_HOG1 terminator
Plasmid#122435PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMST-1313
Plasmid#118076PurposepTEF1-cexA-tCYC1DepositorInsertpTEF1-cexA-tCYC1
ExpressionYeastPromoterpTEF1Available SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Tet
Plasmid#104524PurposeLI resistance gene moduleDepositorInsertTetracycline resistance gene
UseCloning vectorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI S178A
Plasmid#114159PurposeExpresses ClbI S178ADepositorInsertclbI
Tags6xHis and 6xHis-Thrombin siteExpressionBacterialMutationMutated S178 to AlaAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pde1c
Plasmid#105461Purposeto generate a riboprobe for in situ hybridazationDepositorInsertPde1c (Pde1c Mouse)
UseTo ganerate riboprobeAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5084
Plasmid#85105PurposePtrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli.DepositorInsertecfp-KaiC
TagsECFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ040
Plasmid#67758PurposeExpresses C-terminal domain of Chk1 with C-term GFP fluorescent tagDepositorInsertzebrafish Chk1
UseZebrafish mrna injectionTagsGFPExpressionMammalianMutationonly encodes C-term domain (amino acids 288-410)PromoterCMVAvailable SinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD403
Plasmid#63740PurposeContains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184DepositorInsertLT gene probe
ExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
ExpressionMammalianPromoterCAGAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only