Addgene

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9X NFAT-apha-MHC-Luc

Plasmid

#51941

Purpose

9 copies of NFAT sites in minimal alphaMHC-pGL-3

Depositor

Jeffery Molkentin

Article

Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

Inserts

9x NFAT binding site
alpha-MHC

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-NBRE3

Plasmid

#208254

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X NBRE-LUC

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

FerH

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-NurRE3

Plasmid

#208253

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X Nur response element LUC

Use

Tags

No

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1063-luc2

Plasmid

#227109

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_18xTEAD_luc2

Plasmid

#173003

Purpose

firefly luciferase reporter vector with 18x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

18xTEAD-luc2

Use

AAV and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1065-luc2

Plasmid

#227111

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1094-luc2

Plasmid

#227114

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP1500

Plasmid

#16016

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP (CFLAR Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pGL3 Pro E2

Plasmid

#48749

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing E-box2 (-112 to +98)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Gluc-RMA-IRES-EGFP

Plasmid

#189629

Purpose

Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Mutation

Promoter

IRES and hSyn

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pAGH10 - RlucFluc minus 1 HIV-1

Plasmid

#198224

Purpose

Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

Depositor

Isha Jain

Article

Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

Inserts

Renilla luciferase
Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRL-TK 3'FBXW7 UTR

Plasmid

#26649

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Sep 7. ():.

Insert

FBXW7 (FBXW7 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains only the 3'UTR of FBXW7, nucleotide…

Promoter

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only
pMiR-RECK-3'UTR

Plasmid

#53614

Purpose

Luciferase reporter assay for microRNA binding on the RECK 3'UTR

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pKM19

Plasmid

#123655

Purpose

Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

Depositor

Kevin Maringer

Article

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

pIEx

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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1 kb Axin2 basic

Plasmid

#25701

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.24-Cd36-enhancer4

Plasmid

#138576

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag A)

Plasmid

#16605

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag A) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragA (XmnI->KpnI). The KpnI site is at -1194 …

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pAAV_12xTEAD_luc2

Plasmid

#173004

Purpose

firefly luciferase reporter vector with 12x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

12xTEAD-luc2

Use

AAV and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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