We narrowed to 1,706 results for: nif

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pAAV hSyn INTRON mEmerald-STIM1 WPRE

Plasmid

#236234

Purpose

AAV expression of mouse STIM1 internally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Stim1 (Stim1 Mouse)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-FOXC1-T2A-miRFP670

Plasmid

#182336

Purpose

Expresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box C1 (FOXC1 Human)

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SOX2-T2A-miRFP670

Plasmid

#187375

Purpose

Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

SOX2 (SOX2 Human)

Tags

T2A-miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Empty Bridge Control Zfp462-Klf4SE

Plasmid

#127666

Purpose

Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

gRNAs 129, 135, 115, 117

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0625

Plasmid

#186691

Purpose

VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBFC0687

Plasmid

#186692

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-tagBFP-HDR

Plasmid

#163751

Purpose

HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

SOX2 (SOX2 Human)

Use

CRISPR and Synthetic Biology

Tags

P2A-tagBFP

Mutation

Does not contain start codon to avoid random inte…

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBFC0694

Plasmid

#186693

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Target Zfp462-Klf4SE

Plasmid

#127668

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

EF1a and hU6

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-Jph3 WPRE

Plasmid

#236238

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

Plasmid

#236239

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.1 (CACNA1A Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mEmerald-CAPRIN1

Plasmid

#164218

Purpose

Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF-1a

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-LAMP1-HaloTag

Plasmid

#164209

Purpose

Expresses HaloTag tagged LAMP1 under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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