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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 2

Plasmid

#193594

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 2 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLTR PKC beta II

Plasmid

#8417

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCMV DCC mut

Plasmid

#16460

Depositor

Bert Vogelstein

Article

Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

Insert

DCC (DCC Human)

Expression

Mammalian

Mutation

5' end of DCC

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLTR PKC delta

Plasmid

#8419

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
PDLIM7_PDZ_1

Plasmid

#103940

Purpose

Protein expression and purification of ENIGMA PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDLIM7 (PDLIM7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-GSX2-tTA-BleoR

Plasmid

#161748

Purpose

Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Article

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

Advanced tTA

Use

CRISPR

Expression

Mammalian

Promoter

endogenous GSX2 promoter

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pLTR PKC alpha

Plasmid

#8416

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only