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Showing: 4201 - 4250 of 22703 results

  43. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Kevin White
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112295-plasmid-map-sequence-id-228416

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  44. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  45. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160730-plasmid-map-sequence-id-357931

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  46. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid
    #234869
    Purpose
    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.
    Depositor
    Michael Murrell
    Article
    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:
    Insert
    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    Use
    Lentiviral
    Tags
    GFP, RFP, FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234869-plasmid-map-sequence-id-471233

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  47. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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Showing: 4201 - 4250 of 22703 results