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Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK845
Plasmid#219746PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiensDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE
Plasmid#188946PurposeLevel 1 destination vector containing pBBR1 origin of replication and CIDAR AE scarsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
0A-1169
Plasmid#220935PurposeA QUAS line that expresses VSR proteins in Ae. aegypti when crossed with a driver line, Opie-2 dsRed.DepositorInsertCricket paralysis virus (CrPV) 1A protein, Nodamura virus (NoV) B2 protein, and flock house virus (FHV) B2
UseTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP226
Plasmid#222056PurposeGreenGate AE bridge module containing filler sequence.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1 Delta RC
Plasmid#164522PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)DepositorInsertNSP1 Delta RC (ORF1ab )
UseTagsExpressionMammalianMutationInsert of NSP1 gene's CDS from SARS-CoV-2 is…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY0207 ACTB atgRNA with v1 scaffold
Plasmid#179107PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNADepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1 124A/125A
Plasmid#164523PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)DepositorInsertNSP1 124A/125A (ORF1ab )
UseTagsExpressionMammalianMutationInsert of NSP1 gene's CDS from SARS-CoV-2 is…PromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-attp-u6-blank-sgrna-backbone
Plasmid#234076PurposeBlank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 lociDepositorInsertsgRNA-Blank
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX304-KDM6A-M1190T/E1335D
Plasmid#203581PurposeExpresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.DepositorInsertKDM6A (KDM6A Human)
UseLentiviralTagsHA-taggedExpressionMammalianMutationChanged Methionine 1190 Threonine and Glutamic Ac…PromoterCMVAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLfa PUb TAL-KMO-R
Plasmid#52888PurposeRight hand TALEN against Aedes aegypti KMO geneDepositorInsertTALEN-KMO
UseTagsExpressionMutationPromoterAe aegypti polyubiquitinAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomaGCaMP7
Plasmid#182942PurposeCre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SomaGCaMP7
Plasmid#182941PurposeCre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseSynthetic BiologyTagsExpressionMutationPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only