We narrowed to 16,834 results for: EMB

1. GGE254

   Plasmid

   #120789

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE255

   Plasmid

   #120788

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXF262

   Plasmid

   #139038

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   cox-4 (no STOP codon) (W09C5.8 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSDH50

   Plasmid

   #139037

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJF7

   Plasmid

   #139036

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tomm-20 (no STOP codon) (tomm-20 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR Bmi1-C

   Plasmid

   #30511

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   Bmi1 (Bmi1 Mouse)
   

   Use

   Gateway - donor vector

   Tags

   Expression

   Mutation

   NO STOP

   Promoter

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH736-CEN-staHIS3

   Plasmid

   #38228

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   TDH3 (=GPD)

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFusX4-35

   Plasmid

   #63444

   Purpose

   Encodes RVD positions 10-12 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NN NI NN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pSDH66

   Plasmid

   #139053

   Purpose

   MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   unc-54 3'UTR (unc-54 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046181

    Plasmid

    #149090

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pML3

    Plasmid

    #108341

    Purpose

    MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFusX1-31

    Plasmid

    #63249

    Purpose

    Encodes RVD positions 1-3 in TALEN assembly

    Depositor

    Stephen Ekker

    Article

    Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

    Insert

    HD NG NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pFusX4-31

    Plasmid

    #63440

    Purpose

    Encodes RVD positions 10-12 in TALEN assembly

    Depositor

    Stephen Ekker

    Article

    Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

    Insert

    HD NG NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

14. POC1535

    Plasmid

    #226476

    Purpose

    pMOLC360_VL_I1 (Chloramphenicol R) containing the insert I1 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. POC1536

    Plasmid

    #226477

    Purpose

    pMOLC360_VM_I2 (Chloramphenicol R) containing the insert I2 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. POC1537

    Plasmid

    #226478

    Purpose

    pMOLC360_VM_I3 (Chloramphenicol R) containing the insert I3 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. POC1538

    Plasmid

    #226479

    Purpose

    pMOLC360_VR_I4 (Chloramphenicol R) containing the insert I4 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. POC1539

    Plasmid

    #226480

    Purpose

    pMOLC360_VL_I5 (Chloramphenicol R) containing the insert I5 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. POC1540

    Plasmid

    #226481

    Purpose

    pMOLC360_VM_I6 (Chloramphenicol R) containing the insert I6 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. POC1541

    Plasmid

    #226482

    Purpose

    pMOLC360_VR_I7 (Chloramphenicol R) containing the insert I7 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge