We narrowed to 3,326 results for: hwa

Showing: 1681 - 1700 of 3326 results

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VEE-EGFP-IRES-PuroR

Plasmid

#242414

Purpose

Spectral unmixing: EGFP only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

EGFP
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 Y254C

Plasmid

#231587

Purpose

ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed tyrosine 254 to cysteine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Apex2 and FLag

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPPAT-2XStrep

Plasmid

#125620

Purpose

expresses human PPAT protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-globin_del5UTR_PTC39-xrRNA-4H

Plasmid

#108367

Purpose

Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

Depositor

Niels Gehring

Article

Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

Insert

PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites

Expression

Mammalian

Mutation

PTC39

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr4

Plasmid

#108096

Purpose

Expresses mouse Egr4 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKS7107

Plasmid

#89051

Purpose

Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Nm crRNA
Nm tracrRNA
hNmCas9

Use

CRISPR

Tags

HA tag, NLS, and SV40 NLS

Promoter

EF1a and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pAC-aZEA

Plasmid

#53286

Purpose

Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pACSV-Ptc

Plasmid

#37675

Depositor

Philip Beachy

Article

Yao et al Cell. 2006 Apr 21;125(2):343-57.

Insert

ptc (ptc Fly)

Expression

Insect

Promoter

Actin 5C

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
3122 pSFFV-neo Bcl-XL MI-3

Plasmid

#8811

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL MI-3 (BCL2L1 Human)

Expression

Mammalian

Mutation

BH1 WGR-->WAR

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pmiR-96 cluster promoter

Plasmid

#62517

Purpose

microRNA 183-96-182 cluster promoter luciferase plasmid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

Insert

promoter of microRNA-183-96-182 cluster

Tags

RenSP (optimized renilla luciferase gene)

Expression

Mammalian

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pHRIG-PRAS40

Plasmid

#53599

Purpose

lentiviral expression of constitutively active PRAS40 and EGFP

Depositor

Heng Zhao

Article

Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

Insert

PRAS40 (AKT1S1 Human)

Use

Lentiviral

Tags

IRES-EGFP and myc

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (L135V)

Plasmid

#31154

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Leucine 135 changed to Valine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (E87R)

Plasmid

#31152

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glutamic acid 87 changed to Arginine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG1

Plasmid

#23413

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG1 (PRKG1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only