We narrowed to 4,504 results for: pcr

1. pR26-CMVconst

   Plasmid

   #127373

   Purpose

   Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLP700

   Plasmid

   #203742

   Purpose

   C-terminal tagging of target proteins with AID*/3xHA degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain codons 71-114
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNA_DNMT3a-T1

   Plasmid

   #41821

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3a-T1 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA_DNMT3a-T2

   Plasmid

   #41822

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3a-T2 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKnockinDonor-humanCTCF-miniAID-mClover3

   Plasmid

   #136883

   Purpose

   knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

   Depositor

   Chunliang Li

   Article

   Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

   Insert

   CTCF-knockin-homology arm-miniAID-mClover3
   

   Use

   Tags

   miniAID-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA-hIRF-1 #12

   Plasmid

   #61079

   Purpose

   Expresses a guide RNA (gRNA) to target human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   gRNA_hIRF1 promoter #12 (IRF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

   Plasmid

   #86421

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-KAR2SS-GFP1-10
   

   Use

   Tags

   GFP1-10

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

11. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

12. Alt AlphaPS3

    Plasmid

    #14070

    Depositor

    Richard Hynes

    Article

    Stark et al Development. 1997 Nov . 124(22):4583-94.

    Insert

    Drosphila Alt Alpha PS3 Integrin (scb Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. (F) pXYL (G) P1 (SBE216)

    Plasmid

    #195017

    Purpose

    promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pXYL (G)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. (R) pXYL (D) P1 (SBE215)

    Plasmid

    #195016

    Purpose

    promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (R) pXYL (D)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. (R)pADH2(D)_P1(SBE214)

    Plasmid

    #195015

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (B) pADH2 (R)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. (B) NAT marker (R) (SBE173)

    Plasmid

    #195014

    Purpose

    nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+NAT+tNOS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. (S) insD_ku70 (Q) (SBE139)

    Plasmid

    #187627

    Purpose

    the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (S) insD KU70 (Q)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. (P) insUP_ku70 (SBE138) (B)

    Plasmid

    #187626

    Purpose

    the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (P) insUP KU70 (B)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Topo-Tbxas1_ISH_S

    Plasmid

    #170307

    Purpose

    Encoding partial sense strand of the mouse Tbxas1 gene for in situ hybridization.

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    Partial sense strand of the mouse Tbxas1 gene for in situ hybridization
    

    Use

    For ivf

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Topo-Tbxas1_ISH_AS

    Plasmid

    #170308

    Purpose

    Encoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    Partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
    

    Use

    For ivf

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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