We narrowed to 59,686 results for: plasmid
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Plasmid#192250Purposeexpressing sgRNA-2 targeting eIF3b locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
UseCRISPRExpressionMammalianAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b sgRNA-1 PX459 plasmid
Plasmid#192249Purposeexpressing sgRNA-1 targeting eIF3b locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
UseCRISPRExpressionMammalianAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-2 lentiCRISPR v2 plasmid
Plasmid#192232Purposelentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-1 lentiCRISPR v2 plasmid
Plasmid#192231Purposelentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS15A sgRNA-2 lentiCRISPR v2 plasmid
Plasmid#192228Purposelentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTRDepositorInsertsgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
UseCRISPR and LentiviralAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS15A sgRNA-1 lentiCRISPR v2 plasmid
Plasmid#192227Purposelentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTRDepositorInsertsgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
UseCRISPR and LentiviralAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM4896 (pCV0008)
Plasmid#120081PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192239Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3eDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
ExpressionMammalianAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
ExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192241Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3fDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)
ExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY156: SUZ12 px330 sgRNA Cas9 plasmid
Plasmid#170792PurposeThis plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
ExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
Plasmid#101205PurposeBacterial expression plasmid for SpCas9 REC3 domainDepositorInsertSpCas9 variant K506–Q712
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK506–Q712PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant
Plasmid#101202PurposeBacterial expression plasmid for SpCas9∆REC3 variantDepositorInsertSpCas9 variant M1–N497,GGS,V713–D1368
Tags10x His, MBP, and TEV siteExpressionBacterialMutationM1–N497, GGS, V713–D1368PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant
Plasmid#101233PurposeBacterial expression plasmid for SpCas9 Cluster 2 variantDepositorInsertSpCas9 variant G582A/V583A/E584A/D585A/N588A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationG582A, V583A, E584A, D585A and N588APromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sender_plasmid2
Plasmid#140693PurposeEncodes inducible expression of RhlI (C4-HSL quorum sensing synthase)DepositorInsertRhlI (rhlI )
TagsssrA degradation tag (ASV)ExpressionBacterialPromoterengineered lac promoter (IPTG inducible)Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA
Plasmid#20296PurposeCre-activated AAV expression of EYFPDepositorInsertEYFP
UseAAVExpressionMammalianAvailable sinceMay 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 hId1
Plasmid#16061DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertId1 (ID1 Human)
ExpressionMammalianAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MIGR-mFoxP3
Plasmid#24067DepositorAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by