159,333 results

1. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191796

   Purpose

   vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13X
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R84A, H89A, R739A, R740A, H745A, H746A, H747A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX_305

   Plasmid

   #41390

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTurquoise2

   Plasmid

   #198196

   Purpose

   Spectral correction: Expression of mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA6A-EGFR WT

   Plasmid

   #42665

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SYP-HRP

   Plasmid

   #117185

   Purpose

   Plasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   SYP-HRP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NeonGreen

   Plasmid

   #197924

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   NeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag p38 alpha

   Plasmid

   #20351

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 alpha (Mapk14 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL7.0: tgRFPt-SSPB WT

   Plasmid

   #60415

   Purpose

   Mammalian Expression of tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. M13cp-dg3 hp

    Plasmid

    #218095

    Purpose

    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

    Depositor

    Kevin Esvelt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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