164,609 results

1. pETM6

   Plasmid

   #49795

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Use

   Tags

   S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-2E2-HA-mEGFP

   Plasmid

   #129595

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mucolipin1 D471-472K-pHcRed C1

   Plasmid

   #62961

   Purpose

   full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

   Depositor

   Paul Luzio

   Article

   Pryor et al Traffic. 2006 Oct;7(10):1388-98.

   Insert

   Mucolipin-1 (MCOLN1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   D471/472K (and two silent mutations T77 ACA inst…

   Promoter

   CMV

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. CMVTO-Furin

   Plasmid

   #179920

   Purpose

   Furin protein

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Furin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-iT7

   Plasmid

   #211699

   Purpose

   NIS-Seq-compatible lentiviral vector for expressing sgRNAs

   Depositor

   Jonathan Schmid-Burgk

   Article

   Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_LaG16_2_synNotch_Gal4VP64

   Plasmid

   #85421

   Purpose

   Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   LaG16_2 GFP nanobody synNotch Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Use

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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