165,947 results

1. pDB5322

   Plasmid

   #204832

   Purpose

   An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Expression

   Yeast

   Promoter

   CMV promoter and enotetSW4 promoter

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+StayGold-C Cloning Vector

   Plasmid

   #208337

   Purpose

   To express a protein of interest fused to the C-terminus of StayGold in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   StayGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ss-cfSGFP2

   Plasmid

   #37535

   Depositor

   Ikuo Wada

   Article

   Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

   Insert

   ss-cfSGFP2 (cysteine-free SGFP2)
   

   Tags

   signal sequence of α1-antitrypsin (M1-K34)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82706

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

   Plasmid

   #124603

   Purpose

   Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   Soma-localized ChrimsonR
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176273

   Purpose

   Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (sgTet) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK-GFP

   Plasmid

   #66844

   Purpose

   Tet-inducible expression of GFP in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-N1-GalT

   Plasmid

   #87327

   Purpose

   To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ataxin-3 *VBM

    Plasmid

    #89976

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 282RKRR to 282HNHH

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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