2,226 results

1. CaMK2rep

   Plasmid

   #239622

   Purpose

   CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Use

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaMK2rep-S603A

   Plasmid

   #239623

   Purpose

   Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Use

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Mutation

   changed Serine 603 to Alanine

   Promoter

   CMV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nCaMK2rep

   Plasmid

   #239624

   Purpose

   CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nCaMK2rep-S603A

   Plasmid

   #239625

   Purpose

   Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Changed serine 603 to Alanine

   Promoter

   hSyn

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pALN132

   Plasmid

   #239799

   Purpose

   Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain

   Depositor

   Iain Cheeseman, Ally Nguyen

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   MmCENPT--Rn-Histone Fold Domain
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney Murine Leukemia Virus (MMLV) LTR promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pALN148

   Plasmid

   #239800

   Purpose

   Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain and 4 non-synonymous substitutions

   Depositor

   Iain Cheeseman, Ally Nguyen

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mm-CENPT-Rn-Histone Fold Domain-4NS
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   4 nucleotide substitution in rat histone fold dom…

   Promoter

   Moloney Murine Leukemia Virus (MMLV) LTR promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IP-EGFP-PA-BAX(ER, G108V)

   Plasmid

   #233344

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   BAX(3-171), N-terminus and C-terminus are deleted…

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-InPGrp-Kras

   Plasmid

   #236001

   Purpose

   Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

   Insert

   InPGrp-Kras (Cyth3 Synthetic, Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Lyso-InPGrp

   Plasmid

   #236002

   Purpose

   Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

   Depositor

   Jin Zhang

   Article

   Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

   Insert

   Lyso-InPGrp (Cyth3 Synthetic, Rat)
   

   Use

   Tags

   Cerulean, Full-length LAMP1, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Endo-InPGrp

    Plasmid

    #236003

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Endo-InPGrp (Cyth3 Synthetic, Rat)
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

    Plasmid

    #233270

    Purpose

    To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

    Depositor

    Jonathan Ploski, Glenn Schafe

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #5)

    Plasmid

    #233271

    Purpose

    To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

    Depositor

    Jonathan Ploski, Glenn Schafe

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDsRed1-CMV-Rat Npas4-DsRed1-pA

    Plasmid

    #233272

    Purpose

    To Express a Rat NPAS4-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski, Glenn Schafe

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    rat Npas4 DsRed fusion (Npas4 Rat)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Y2D

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1)

    Plasmid

    #233273

    Purpose

    To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

    Depositor

    Jonathan Ploski, Glenn Schafe

    Insert

    GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)

    Plasmid

    #233274

    Purpose

    To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

    Depositor

    Jonathan Ploski, Glenn Schafe

    Insert

    GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDsRed1-CMV-Rat Nurr1-DsRed1-pA

    Plasmid

    #233275

    Purpose

    To Express a Rat Nurr1-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski, Glenn Schafe

    Insert

    Nurr1 (Nr4a2 Rat)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    P2A

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-pSyn_R-PTEN

    Plasmid

    #227437

    Purpose

    Expression of the R-PTEN sensor under the Synapsin promoter in an AAV backbone

    Depositor

    Tal Laviv

    Article

    Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

    Insert

    R-PTEN sensor (Pten Rat)
    

    Use

    AAV

    Tags

    mCyRFP2 and mMaroon

    Expression

    Mammalian

    Mutation

    R14G

    Promoter

    Synapsin

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE-RnCENP-W

    Plasmid

    #238064

    Purpose

    Expresses rat CENP-W; untagged; made for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Rat CENP-W (Cenpw Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFPc1 QFR IP3R3

    Plasmid

    #236454

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q-FR/A-AA

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK41

    Plasmid

    #236110

    Purpose

    Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody

    Depositor

    Shinsuke Niwa

    Article

    Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb

    Inserts

    KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
    KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
    

    Use

    Tags

    ALFA tag nanobody, His tag, SNAP tag, and StrepII…

    Expression

    Bacterial

    Mutation

    C7S mutation is introduced. 1-430 aa is encoded.

    Promoter

    T7

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge