We narrowed to 21,800 results for: Sis

1. pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter

   Plasmid

   #211847

   Purpose

   Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   R181A R185A

   Promoter

   35S

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 NKX6-2-Venus S246A/T252A

   Plasmid

   #209180

   Purpose

   Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (S246A/T252A) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (S246A/T252D)

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 NKX6-2-Venus Y238F

   Plasmid

   #209181

   Purpose

   Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (Y238F) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (Y238F)

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB1-C-TelR

   Plasmid

   #204981

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMB1-D-TelR

   Plasmid

   #204982

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMB1-E-TelR

   Plasmid

   #204984

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMB2a-TelR

   Plasmid

   #204985

   Purpose

   Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMB2b-TelR

   Plasmid

   #204986

   Purpose

   Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMB2-A-TelR

   Plasmid

   #204987

   Purpose

   Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMB2-B-TelR

    Plasmid

    #204988

    Purpose

    Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB2-C-TelR

    Plasmid

    #204989

    Purpose

    Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMB2-D-TelR

    Plasmid

    #204990

    Purpose

    Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMB3-A-TelR

    Plasmid

    #204991

    Purpose

    Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RS02060_pMQ30_KO_Kan

    Plasmid

    #185391

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS02060 upstream flanking homology region
    RR42_RS02060 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RS28935_pMQ30_KO_Kan

    Plasmid

    #185392

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS28935 upstream flanking homology region
    RR42_RS28935 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RS18965_pMQ30_KO_Kan

    Plasmid

    #185394

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18965 upstream flanking homology region
    RR42_RS18965 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RS18970_pMQ30_KO_Kan

    Plasmid

    #185395

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18970 upstream flanking homology region
    RR42_RS18970 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-MD-mTurquoise

    Plasmid

    #193094

    Purpose

    constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    MD-mTurquoise
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1BR9-AtWUS

    Plasmid

    #193865

    Purpose

    E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized
    

    Use

    Tags

    6xHis, GFP11, and R9

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCble

    Plasmid

    #185861

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNB201

    Plasmid

    #169112

    Purpose

    TESEC expression vector for Alr from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    alr (alr Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNB205

    Plasmid

    #169116

    Purpose

    TESEC expression vector for Asd from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    asd (asd Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNB206

    Plasmid

    #169117

    Purpose

    TESEC expression vector for CysH from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    cysH (cysH Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNB207

    Plasmid

    #169118

    Purpose

    TESEC expression vector for DapB from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    dapB (dapB Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNB208

    Plasmid

    #169119

    Purpose

    TESEC expression vector for TrpD from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    trpD (trpD Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CDS_Cas9-NLS

    Plasmid

    #136121

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_AtcoCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS-synPCB2.2

    Plasmid

    #139479

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V554L

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNOC-CRISPR-compact

    Plasmid

    #99369

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMCh-1478

    Plasmid

    #82424

    Purpose

    Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR-IAA28_no stop

    Plasmid

    #83861

    Purpose

    Gateway donor vector for IAA28 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NP_568478.1 (IAA28 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTAct2 (GB0210)

    Plasmid

    #68191

    Purpose

    Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p5'UTRAct2 (GB0193)

    Plasmid

    #68178

    Purpose

    Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    5'UTR AtAct2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Ac 1.8kb red opsin GFP.

    Plasmid

    #72915

    Purpose

    red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFP

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    red opsin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ac 1.8kb red opsin DsRed

    Plasmid

    #72914

    Purpose

    red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRed

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    red opsin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET23a-MmUBB(SnaBI)

    Plasmid

    #69558

    Purpose

    In vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The two last codons (TATTAA; encoding the last ty…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pET23a-MmUBB(Y305P)

    Plasmid

    #69557

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon TAT encoding tyrosine 305 was changed to CC…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JW776

    Plasmid

    #64817

    Purpose

    BiFC rCUC2

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    N-terminal domain Firefly

    Expression

    Mutation

    rCUC2

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge