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pDONR223_MDM2_WT

Plasmid

#82897

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC52A3

Plasmid

#132181

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_STAT3_p.Y705F

Plasmid

#82086

Purpose

Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway entry vector

Mutation

Y705F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227896

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBFB_WT

Plasmid

#81929

Purpose

Gateway Donor vector containing CBFB, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CBFB (CBFB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway entry vector

Mutation

V617F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35F6

Plasmid

#132073

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F6 (C2orf18 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227904

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_WT

Plasmid

#81734

Purpose

Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_WT

Plasmid

#82275

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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