We narrowed to 6,480 results for: poly

1. pGL4_10xUAS-CMVmin-BC0432-luc2

   Plasmid

   #227124

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0432

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0432 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL4_10xUAS-CMVmin-BC0434-luc2

   Plasmid

   #227125

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0434

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0434 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4_10xUAS-CMVmin-BC0407-luc2

   Plasmid

   #227119

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0407

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0407 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4_10xUAS-CMVmin-BC0436-luc2

   Plasmid

   #227126

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0436

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0436 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL4_10xUAS-CMVmin-BC0400-luc2

   Plasmid

   #227117

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0400

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0400 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4_10xUAS-CMVmin-BC0439-luc2

   Plasmid

   #227127

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0439

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0439 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4_10xUAS-CMVmin-BC0396-luc2

   Plasmid

   #227115

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4_10xUAS-CMVmin-BC0397-luc2

   Plasmid

   #227116

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0397

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0397 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4_10xUAS-CMVmin-BC0431-luc2

   Plasmid

   #227123

   Purpose

   Barcoded assay, UAS sensor for split TEV assays; barcode BC0431

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   10x clustered UAS element linked to CMV minimal promoter driving barcode 0431 and a luciferase reporter gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBK_Gal4-DBD-Ar

    Plasmid

    #227098

    Purpose

    Ar receptor overexpression for luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    Ar (Ar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4_10xUAS-CMVmin-BC0443-luc2

    Plasmid

    #227131

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0443

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0443 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4_CRE-CMVmin-BC0481-luc2

    Plasmid

    #227132

    Purpose

    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0481

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered CRE element linked to CMV minimal promoter driving barcode 0481 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4_10xUAS-CMVmin-BC0411-luc2

    Plasmid

    #227122

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0411

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0411 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4_10xUAS-CMVmin-BC0405-luc2

    Plasmid

    #227118

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0405

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0405 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4_10xUAS-CMVmin-BC0408-luc2

    Plasmid

    #227120

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0408

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0408 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4_10xUAS-CMVmin-BC0441-luc2

    Plasmid

    #227129

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0441

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0441 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4_CRE-CMVmin-BC0498-luc2

    Plasmid

    #227134

    Purpose

    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0498

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered CRE element linked to CMV minimal promoter driving barcode 0498 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4_10xUAS-CMVmin-BC0440-luc2

    Plasmid

    #227128

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0440

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0440 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4_NFAT-RE-CMVmin-BC0524-luc2

    Plasmid

    #227139

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC0524

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4_CRE-CMVmin-BC0504-luc2

    Plasmid

    #227135

    Purpose

    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0504

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered CRE element linked to CMV minimal promoter driving barcode 0504 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL4_10xUAS-CMVmin-BC0442-luc2

    Plasmid

    #227130

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0442

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0442 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPSt_35Spr-GFP-t35S

    Plasmid

    #203592

    Purpose

    Fully assembled Plant STARR-seq plasmid with the 35S terminator.

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Inserts

    CaMV 35S promoter + Zm00001d041672 5'UTR
    eGFP
    CaMV 35S terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIp128-CUP1-His6-CUbo(K48R)-GFP

    Plasmid

    #212793

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo(K48R)-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    CUb(K48R)

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIp128-CUP1-His6-CUbo(K63R)-GFP

    Plasmid

    #212794

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo(K63R)-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    CUb(K63R)

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

    Plasmid

    #212747

    Purpose

    Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48) aa24-150
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Cue1 ΔU7BD

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

    Plasmid

    #212738

    Purpose

    Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    HOIP(C885A)

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SUMO-Twinkle 43-372

    Plasmid

    #205054

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks Mitochondrial localization signal and C ter…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV6-AN-HA_aa 321-843 REV1

    Plasmid

    #201587

    Purpose

    Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA repair protein REV1 (REV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 321-843 of DNA repair prote…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

    Plasmid

    #201545

    Purpose

    Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks N-terminal residues 1-79

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid

    #201546

    Purpose

    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 188-228

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

    Plasmid

    #201548

    Purpose

    Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks C-terminal residues 364-409

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET19-TdTdelta5cys

    Plasmid

    #207465

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTdelta5cys (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-3UTR

    Plasmid

    #202550

    Purpose

    Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media

    Depositor

    Rostislav Skrabana

    Article

    Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.

    Type

    Empty backbone

    Use

    Tags

    ATG initiation codon followed by an IGKV3 signal …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. W118-1_hSTK16-Flag

    Plasmid

    #180403

    Purpose

    lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    STK16 (STK16 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. W118-1_Flag-hSRPK3

    Plasmid

    #180404

    Purpose

    lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    SRPK3 (SRPK3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAV.CBA.YFP.miR-E_shStk16-2

    Plasmid

    #180392

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-2 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV.CBA.YFP.miR-E_shStk16-3

    Plasmid

    #180393

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-3 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV.CBA.YFP.miR-E_shTgm5-1

    Plasmid

    #180394

    Purpose

    Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shTgm5-1 (Tgm5 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AAV.CBA.YFP.miR-E_shTgm5-2

    Plasmid

    #180395

    Purpose

    Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shTgm5-2 (Tgm5 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. AAV.CBA.YFP.miR-E_shTgm5-3

    Plasmid

    #180396

    Purpose

    Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shTgm5-3 (Tgm5 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pGEX4T3-GST-PolB(K72A)

    Plasmid

    #177132

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMT-FLAG-CBPΔ(N+HAT)

    Plasmid

    #182841

    Purpose

    An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP (w Fly)
    

    Use

    Tags

    FLAG tag

    Expression

    Insect

    Mutation

    dletion of residues 1566-2142 from CBP1476-3222

    Promoter

    MT

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLVX-Flag-PolB(T304I)-Puro

    Plasmid

    #177143

    Purpose

    Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGEX4T3-GST-PolB(K206A/K244A)

    Plasmid

    #177135

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-DEST42 -SSRP1

    Plasmid

    #170439

    Purpose

    Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    ssrp1 (Ssrp Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. CPH3486 (YCp LEU2 Rpb1 F1492A)

    Plasmid

    #91808

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed phenylalanine 1492 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. CPH3487 (YCp LEU2 Rpb1 S1493A)

    Plasmid

    #91809

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed serine 1493 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge