We narrowed to 15,797 results for: form

1. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn-ERT2-iCre-ERT2

   Plasmid

   #178059

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin 1 (Syn) promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 10279-M28-663

   Plasmid

   #225662

   Purpose

   Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-UBC-ERT2-iCre-ERT2

   Plasmid

   #178060

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQFlag-USP7 CS puroR

   Plasmid

   #46752

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-Sumo

   Plasmid

   #106980

   Purpose

   LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

   Depositor

   Han Choe

   Article

   Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

   Type

   Empty backbone

   Use

   Tags

   His8-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSKC12 - DelQ

   Plasmid

   #110493

   Purpose

   Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

   Depositor

   Shankar Balasubramanian

   Article

   Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

   Insert

   NRAS 5'- DelQ (bp 30-254) (NRAS Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mutation

   Deletion of the first 29 base pairs of the NRAS 5…

   Promoter

   T7

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-cofilin-S3A

   Plasmid

   #78296

   Purpose

   Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3A

   Promoter

   CAG

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQHA-USP7 CS puroR

   Plasmid

   #46754

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1 blast tBID-flag

    Plasmid

    #211533

    Purpose

    Expresses truncated BID upon doxycycline addition to trigger apoptosis

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    tBID-flag (BID Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated (CASP8) form (p15)

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid

    #88879

    Purpose

    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP fusion protein and HA tag

    Expression

    Mammalian

    Mutation

    amino acids 4-460 only; L78P & L287P *see bel…

    Promoter

    SFFV (spleen focus forming virus)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM656

    Plasmid

    #53086

    Purpose

    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEAT8-137 M1V

    Plasmid

    #173807

    Purpose

    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

    Depositor

    Anne Gershenson

    Article

    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

    Insert

    SERPiNA1 (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1M Otherwise this plasmid encodes the most commo…

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Use

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter/lac operator element

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5d.SFFV.eGFP-miR30n

    Plasmid

    #90333

    Purpose

    miR30 based shMir knockdown

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    eGFP
    na
    SFFV
    miR30n
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. beta catenin binding mutant of alpha catenin sensor

    Plasmid

    #71711

    Purpose

    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin Δβ-cat mutant FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 48- 163

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1 hSCN5A-L325R

    Plasmid

    #145373

    Purpose

    expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 325 to Arginine

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUB6-HuMGF

    Plasmid

    #190782

    Purpose

    Expresses membrane-bound form of human SCF in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    human stem cell factor (KITLG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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