We narrowed to 16,109 results for: form

1. TagBFP(S2h)3(S4h)3-gs-P6f

   Plasmid

   #228083

   Purpose

   Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   BFP, S2h, S4h, P6f
   

   Expression

   Mammalian

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Q54A

   Plasmid

   #217912

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP Q54A
   

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Glutamine at position 55 and 267 mutated to Alani…

   Promoter

   T5

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. T40A

   Plasmid

   #217911

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP T40A
   

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Threonine at position 41 and 253 mutated to Alani…

   Promoter

   T5

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA265

   Plasmid

   #215967

   Purpose

   Fragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SFFV_v1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASt-Xbp1-HA-GFP-3'UTR Spl

   Plasmid

   #201246

   Purpose

   Xbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced form

   Depositor

   Pedro Domingos

   Article

   Cairrao et al Nat Commun. 2022 Mar 24;13(1):1587. doi:

   Insert

   dXbp1 (Xbp1 Fly)
   

   Expression

   Insect

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

   Plasmid

   #191631

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase (R506S mutation, inactive form)
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBtPIPLC_N168C His

   Plasmid

   #173809

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Asn168Cys for labeling

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-10xMS2 no wild type

   Plasmid

   #158204

   Purpose

   Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of MS2 without the WT sequence from oligo library experiment
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-10xQb no wild type

   Plasmid

   #158205

   Purpose

   Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLB(N)CX-FLAG-CH3 Del33

    Plasmid

    #133724

    Purpose

    Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

    Depositor

    James A. DeCaprio

    Article

    Borger et al J Virol. 2006 May . 80(9):4292-303.

    Insert

    EP300 (EP300 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    partial (not complete) p300 fragment, AT and CH3 …

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLB(N)CX AT-CH3-FLAG-HA WY

    Plasmid

    #133725

    Purpose

    Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).

    Depositor

    James A. DeCaprio

    Article

    Borger et al J Virol. 2006 May . 80(9):4292-303.

    Insert

    EP300 (EP300 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Mutation

    partial (not complete) p300 fragment, AT and CH3 …

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-Hemmar-DSCP-ftz-FLP(D)

    Plasmid

    #119894

    Purpose

    FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Inserts

    FLP(D)
    DSCP-ftz (ftz Fly)
    

    Use

    Drosophila expression

    Mutation

    a mutated form of FLP, which at position 5 contai…

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-beta2(616-951)Y815A

    Plasmid

    #100744

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform with Y815A mutation

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

    Plasmid

    #80325

    Purpose

    Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

    Depositor

    Daniel Schoenberg

    Article

    Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

    Insert

    PXDNL-003 (PXDNL Human)
    

    Tags

    Biotin

    Expression

    Mammalian

    Mutation

    H170A and H256A

    Promoter

    CMV

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. NET39 Short pmRFP-N2 (451)

    Plasmid

    #61981

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET39 (PPAPDC3 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Short form of NET39

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pIMAY

    Plasmid

    #68939

    Purpose

    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

    Depositor

    Tim Foster

    Article

    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

    Type

    Empty backbone

    Use

    Staphylococcal allelic exchange vector

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FmnI IS04 cDNA/pcDNA3

    Plasmid

    #19321

    Depositor

    Philip Leder

    Article

    Dettenhofer et al PLoS ONE. 2008 . 3(6):e2497.

    Insert

    FmnI Iso4 cDNA (Fmn1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pET29b-pvdF WT

    Plasmid

    #187974

    Purpose

    Expresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Kenjic et al Arch Biochem Biophys. 2019 Mar 30;664:40

    Insert

    pvdF WT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK040 (pET-PurQ-A128T)

    Plasmid

    #73840

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Mutation

    A128T mutant

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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