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LentiCRISPRv2-bleo-PTGER2

Plasmid

#184724

Purpose

EP2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-bleo-PLA2G4A_3

Plasmid

#184725

Purpose

cPLA2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog cPLA2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-PTGER4

Plasmid

#184726

Purpose

EP4-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP4 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-3

Plasmid

#184727

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-4

Plasmid

#184728

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-3

Plasmid

#184729

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-iRFP670-P2Av3-RGECO1.0

Plasmid

#184713

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT004-1x FLAG-HaloTag-G

Plasmid

#202495

Purpose

Express "G" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Geranylgeranyl site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK128

Plasmid

#200140

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Ubc6-TA (233-250)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK127

Plasmid

#200139

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Fox2-PTS1 (870-900)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_33_PyruPro_X2site

Plasmid

#194429

Purpose

Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruPro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_34_EthPro_X2site

Plasmid

#194430

Purpose

Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

EthPro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK123

Plasmid

#200130

Purpose

Type 7 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pRS CEN/ARS

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-133-HiBiT

Plasmid

#198313

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-133-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-289-HiBiT

Plasmid

#198311

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-289-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-190-HiBiT

Plasmid

#198312

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-190-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15

Plasmid

#198284

Purpose

Produces PVCs lacking payloads by elimination of the payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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