We narrowed to 2,306 results for: puc19

1. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #178087

   Purpose

   HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   mScarlet-I mTOR signaling indicator (TOSI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TRE-GNL

   Plasmid

   #204499

   Purpose

   Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   Glis1 (Glis1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPAP_BGL1

   Plasmid

   #183735

   Purpose

   Expresses Aspergillus aculeatus β-glucosidase (BGL1)

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   β-glucosidase (AaBGL1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1 promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTW072M

   Plasmid

   #115960

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl2 (nsP2 Q739L)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   nsP2 Q739L

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS133

   Plasmid

   #211504

   Purpose

   AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   mTagBF2
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS134

   Plasmid

   #211505

   Purpose

   AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   Venus
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS132

   Plasmid

   #211503

   Purpose

   AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   mCherry
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL728

   Plasmid

   #140398

   Purpose

   Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-csoO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL716

    Plasmid

    #140386

    Purpose

    Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-mphO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HBT-GCaMP6-HA

    Plasmid

    #107433

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    GCaMP6S
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15547_PEmax-SuperFi-Cas9

    Plasmid

    #184377

    Purpose

    Mammalian expression of SuperFi-Cas9 PEmax prime editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    PEmax-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-myc NLS

    Expression

    Mammalian

    Mutation

    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBL724

    Plasmid

    #140394

    Purpose

    Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-hucO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

    Plasmid

    #211906

    Purpose

    Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    DARIC-CD22-BBz
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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