Addgene

We narrowed to 2,419 results for: GEM

Showing: 1741 - 1760 of 2419 results

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pSB1A3_GFP_dT19

Plasmid

#89661

Purpose

expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT19

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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C150

Plasmid

#174350

Purpose

Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eIF4G1

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Use

Tags

Expression

Bacterial

Mutation

Promoter

native phage promoters

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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HP4_M13

Plasmid

#120340

Purpose

Helper plasmid for production of phagemid ssDNA, based on m13mp18

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13 phage genome

Use

Tags

Expression

Bacterial

Mutation

Promoter

native phage promoters

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Otx1

Plasmid

#84315

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Otx1 (Otx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLH116

Plasmid

#22651

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1987 Mar . 84(

Insert

Proteolipid Protein (Plp1 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pG5ElbLuc

Plasmid

#89341

Purpose

The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.

Depositor

Roger Davis

Article

Seth et al J Biol Chem. 1992 Dec 5;267(34):24796-80

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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DDX20A

Plasmid

#42376

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DDX20A (DDX20 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJB010

Plasmid

#22616

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (PLP1 Human)

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Pknox2

Plasmid

#74354

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pknox2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx/knotted 1 homeobox 2 (Pknox2 )

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy2d-1

Plasmid

#105459

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Gucy2d (Gucy2d Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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XP1 Xwnt-1

Plasmid

#16990

Depositor

Randall Moon

Article

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-1 (wnt1.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Plcb2

Plasmid

#105991

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Plcb2 (Plcb2 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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