We narrowed to 2,463 results for: T2A

1. pGH484

   Plasmid

   #230838

   Purpose

   Ef1a-AID*-dCas9-T2A-tagBFP-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   AID*-dCas9
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGH915

   Plasmid

   #230843

   Purpose

   Ef1a-BE3-T2A-tagBFP-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   BE3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGH903

   Plasmid

   #230857

   Purpose

   Ef1a-PmCDA1-BE3-T2A-BlastR-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   PmCDA1-BE3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH880

   Plasmid

   #230839

   Purpose

   Ef1a-dCas9-AID*-T2A-tagBFP-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   dCas9-AID*
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGH720

   Plasmid

   #230845

   Purpose

   Ef1a-ABE7.10-T2A-tagBFP-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGH1110

   Plasmid

   #223682

   Purpose

   U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

   Depositor

   Gaelen Hess

   Article

   Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

   Plasmid

   #73164

   Purpose

   multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

   Depositor

   Bruce Edgar

   Article

   Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

   Insert

   mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
   

   Tags

   mRFP1, GFP

   Expression

   Insect

   Mutation

   Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-eGFP-2A-mCherry_D4H

   Plasmid

   #194875

   Purpose

   Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-D4H
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ11434

    Plasmid

    #183625

    Purpose

    pHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    Cas12 Guide (crGFP*)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYS17

    Plasmid

    #202083

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    NT PuroR-T2A-mNG Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p140Tol2-Olactb:SABER

    Plasmid

    #226829

    Purpose

    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    BFP-SABER
    mCherry-t2A-CreERT2
    

    Use

    Zebrafish expression

    Promoter

    Olactb and hsp70

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TC374

    Plasmid

    #164886

    Purpose

    Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rpl28-rtTA(Q)
    

    Expression

    Worm

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYS16

    Plasmid

    #202082

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT mNG-T2A-PuroR Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYS29

    Plasmid

    #202086

    Purpose

    Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    NT HygR-T2A-Halo Minicircle Parent
    

    Use

    CRISPR

    Tags

    HaloTag

    Promoter

    None

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-2A-TurboGFP-PEST

    Plasmid

    #67180

    Purpose

    CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    T2A-TurboGFP-PEST
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYS28

    Plasmid

    #202085

    Purpose

    Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT Halo-T2A-HygR Minicircle Parent
    

    Use

    CRISPR

    Tags

    HaloTag

    Promoter

    None

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-eGFP-2A-mCherry_YDA

    Plasmid

    #194876

    Purpose

    Ratiometric YDA sensor for in vivo neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-YDA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHACK-GAL4>QF2(newHA1)

    Plasmid

    #104873

    Purpose

    HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    T2A-QF2
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ac5-STABLE1-blast

    Plasmid

    #208033

    Purpose

    Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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