We narrowed to 2,278 results for: Tub;

1. pCMV_SEPT9_i1 D1-25-msfGFP

   Plasmid

   #180333

   Purpose

   mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 D1-25

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

   Plasmid

   #107730

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pME-GcAMP5

   Plasmid

   #126569

   Purpose

   Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR3-vsv-GCN4-dCEN-INCENP

   Plasmid

   #108510

   Purpose

   expression of vsv-GCN4-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   GCN4-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   replace CEN box for GCN4 dimerazation domain

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBrain-GFP-TACC3KDP(S558A)-shTACC3

   Plasmid

   #59357

   Purpose

   Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC/RSV-rat Agt

   Plasmid

   #122104

   Purpose

   Expression of angiotensinogen

   Depositor

   John Chan

   Article

   Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

   Insert

   angiotensinogen (Agt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV_SEPT2 NCmut-mApple

   Plasmid

   #180321

   Purpose

   mammalian expression of human SEPT2 NCmut fused to monomeric Apple

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   SEPT2 F20D, V27D

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV_SEPT9_i1-i5-msfGFP

    Plasmid

    #180332

    Purpose

    mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1-i5

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLAP-MIS12-KARD

    Plasmid

    #114057

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-mCGN-mCGNL1-Rod1-Myc

    Plasmid

    #236526

    Purpose

    For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGN-CGNL1 (Cgn Mouse)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Str-KDEL_SBP-EGFP-ColX

    Plasmid

    #222305

    Purpose

    Synchronize the trafficking of CollagenX from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide of COL10A1 removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Str-KDEL_SBP-EGFP-gp135

    Plasmid

    #222306

    Purpose

    Synchronize the trafficking of gp135/podocalyxin from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

    Plasmid

    #212108

    Purpose

    Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

    Insert

    GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of both GABARAPs

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

    Plasmid

    #196876

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196883

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge