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pCAG-Myc-KATNB1

Plasmid

#160054

Purpose

Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNB1 (KATNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL2 residues 1-98

Plasmid

#180619

Purpose

Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL2 (KATNAL2 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-98

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KC1 MIT, residues 235-316

Plasmid

#180622

Purpose

Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 235-316

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBtPIPLC_N168C His

Plasmid

#173809

Purpose

Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Mary F Roberts, Anne Gershenson

Article

He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

Insert

Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase

Use

Tags

6XHis

Expression

Bacterial

Mutation

Asn168Cys for labeling

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Unc5c.a-AP-His

Plasmid

#72054

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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plko-tet-on puromycin-shMTH1

Plasmid

#110918

Purpose

To knock down MTH1 (Mut T Homolog 1) after addition of doxycline

Depositor

Priyamvada Rai

Article

Patel et al Oncogene. 2015 May 14;34(20):2586-96. do

Insert

MTH1 (NUDT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO-BROX 1-401

Plasmid

#89866

Purpose

Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Use

Tags

His Tag, V5 tag

Expression

Bacterial

Mutation

expresses only residues 1-401

Promoter

T7

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Sdk2-Fc-His

Plasmid

#72135

Purpose

Expresses the extracellular region of the Sdk2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sdk2 (Sdk2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(S)-Fc-His

Plasmid

#72152

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-Fc-His

Plasmid

#72153

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5a.a-AP-His

Plasmid

#72050

Purpose

Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a.a (Unc5a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Rgma-Fc-His

Plasmid

#72133

Purpose

Expresses the extracellular region of the RGMa protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Rgma (Rgma Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-AP-His

Plasmid

#72027

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3d(S)-Fc-His

Plasmid

#72144

Purpose

Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6a.z-AP-His

Plasmid

#72038

Purpose

Expresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.z (Sema6a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5d.y-Fc-His

Plasmid

#72184

Purpose

Expresses the extracellular region of the Unc5D, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.y (Unc5d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.x-AP-His

Plasmid

#72047

Purpose

Expresses the extracellular region of the Sema6D, isoform x protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.x (Sema6d Human, Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.z-AP-His

Plasmid

#72056

Purpose

Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.z (Unc5c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.6-AP-His

Plasmid

#72046

Purpose

Expresses the extracellular region of the Sema6D, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.6 (Sema6d Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge