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Plasmid#99619PurposeTo clone gene of interest downstream of FRT1-Mb2YFP-2A cassetteDepositorInsertMb2EYFP
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT2-Mb2Tomato-2A (IG154)
Plasmid#99620PurposeTo clone gene of interest downstream of FRT2-Mb2Tomato-2A cassetteDepositorInsertMb2Tomato
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT3-HA-H2B-Cerulean-2A (IG158))
Plasmid#99624PurposeTo clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassetteDepositorInsertHA-H2B-Cerulean
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Gb2
Plasmid#25744PurposeControl lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertGb2 (Gnb2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-P2A-Puro
Plasmid#110852PurposeLentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant RhoA 3'UTR
Plasmid#26090DepositorInsertmutant RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationBoth miR-31 binding sites in 3’ UTR mutagenized. …PromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXA1-T2A-eGFP
Plasmid#182337PurposeExpresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXA1-T2A-miRFP670
Plasmid#182335PurposeExpresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5-Cys3A
Plasmid#222313PurposeSynchronize the trafficking of CCR5-Cys3A from the ER.DepositorInsertStreptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
UseTagsExpressionMammalianMutationC321A, C323A, C324APromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAM-DNMT3A-inactive+sgGFP
Plasmid#213169PurposeVector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.DepositorInsertsgGFP
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterE1Fa and U6Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
SAM-DNMT3A-inactive+sgAAVS1_145
Plasmid#213170PurposeVector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.DepositorInsertsgAAVS1_145
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterE1Fa and U6Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-mProser1-FLAG-IRES-Blast
Plasmid#226173PurposeExpresses full length mouse PROSER1 with 2X C-terminal FLAG tagsDepositorInsertProline Serine Rich protein 1 (Proser1 Mouse)
UseTags2X FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_hnRNPA1_YtoW
Plasmid#224253PurposeBacterial expression of N-terminally 6His-tagged hnRNPA1_YtoWDepositorInserthnRNPA1_YtoW (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationY52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129WPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1_oROS-HT_LF(C199S)
Plasmid#216412PurposeExpresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-HT_WPRE
Plasmid#216414PurposeEncodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
EXT1- D164A-pLJC2-3XFLAG
Plasmid#215828PurposeEctopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cellsDepositorInsertExostosin glycosyltransferase 1 Mutant (EXT1 Human)
UseLentiviralTags3X FlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-Dlx-SynaptoTAG
Plasmid#210729PurposeAAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.DepositorInsertEGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Rat, Synthetic)
UseAAVTagsExpressionMutationPromoterDlxAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 WWE-PARP
Plasmid#192548PurposepENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
UseTagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Dcst1-3xHA
Plasmid#183540PurposeExpress Dcst1-3xHA under the mouse Clgn promoter.DepositorInsertDC-STAMP domain containing 1 (Dcst1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291D
Plasmid#115193PurposeLentiviral transduction and expression of PDHA2S291D into any mammalian cellDepositorInsertPDHA2S291D (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291DPromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S293D
Plasmid#115194PurposeLentiviral transduction and expression of PDHA2S293D into any mammalian cellDepositorInsertPDHA2S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S293DPromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291D/S293D
Plasmid#115195PurposeLentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cellDepositorInsertPDHA2S291D/S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291D/S293DPromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Dcst2-3xHA
Plasmid#183541PurposeExpress Dcst2-3xHA under the mouse Clgn promoter.DepositorInsertDC-STAMP domain containing 2 (Dcst2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c022
Plasmid#175490PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.DepositorInsertSYK (SYK Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c028
Plasmid#175470PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationH288APromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c021
Plasmid#175468PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationL281RPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c001
Plasmid#175466PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only