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We narrowed to 4,673 results for: pcr

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Showing: 1761 - 1780 of 4673 results

  1. pFA6a-TRP1-PGAL1-HBTH

    Plasmid
    #26898
    Depositor
    Peter Kaiser
    Insert
    HBTH tag N-terminal
    Use
    Pcr tagging yeast
    Available since
    Jan. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  2. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55760-plasmid-map-sequence-id-115314

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  5. pR26-CMVconst-mBMP7

    Plasmid
    #127375
    Purpose
    Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMP7 (Bmp7 Mouse)
    Use
    CRISPR and Mouse Targeting
    Promoter
    CMV enhancer/promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127375-plasmid-map-sequence-id-247999

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  7. pKnockinDonor-humanCTCF-miniAID-mClover3

    Plasmid
    #136883
    Purpose
    knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF
    Depositor
    Chunliang Li
    Publication
    Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CTCF-knockin-homology arm-miniAID-mClover3
    Tags
    miniAID-mClover3
    Expression
    Mammalian
    Promoter
    no promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136883-plasmid-map-sequence-id-310780

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  10. HCA1-Tango

    Plasmid
    #66395
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HCA1 (HCAR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Publication
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway Cloning
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  16. pLO103

    Plasmid
    #90223
    Purpose
    Complements mutations in Aspergillus nidulans gene pyrG (AN6157) (mutants require uridine and uracil)
    Depositor
    Berl Oakley
    Publication
    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATET_09675
    Use
    Aspergillus
    Promoter
    pLac
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90223-plasmid-map-sequence-id-177628

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  18. GPR151-Tango

    Plasmid
    #66327
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR151 (GPR151 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    66327-plasmid-map-sequence-id-344663

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  19. FAB(PX-LD)

    Plasmid
    #214426
    Purpose
    Detection of peroxisome-lipid droplet contact site
    Depositor
    Chi-Lun Chang
    Publication
    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-V5
    Tags
    CFAST-Halo and FKBP-V5-RspAN
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214426-plasmid-map-sequence-id-426360

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  20. pR26-mBMP7

    Plasmid
    #127374
    Purpose
    Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMP7 (Bmp7 Mouse)
    Use
    CRISPR and Mouse Targeting
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127374-plasmid-map-sequence-id-247972

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Showing: 1761 - 1780 of 4673 results