We narrowed to 4,921 results for: puc

1. pBM16

   Plasmid

   #45344

   Depositor

   Brendan Cormack

   Article

   Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

   Type

   Empty backbone

   Use

   C. glabrata expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINER2R

   Plasmid

   #185882

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT643

   Plasmid

   #8610

   Depositor

   Robert Hartley

   Article

   Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

   Insert

   barstar A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two cysteines of barstar changed to alanine, C40A…

   Promoter

   Available since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7-U1

   Plasmid

   #111334

   Purpose

   for in vitro snRNA transcription

   Depositor

   Christine Guthrie

   Article

   Ghetti et al RNA. 1995 Apr;1(2):132-45.

   Insert

   snR19
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW8-aid-3HA-eDSR-Turg1:kanMX6

   Plasmid

   #169363

   Purpose

   C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   3XHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAW8-aid-5Myc-eDSR-Turg1:kanMX6

   Plasmid

   #169364

   Purpose

   C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   5XMyc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNC891

   Plasmid

   #20397

   Depositor

   Beverly Errede

   Article

   Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

   Insert

   YFP-reporter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   X=M (see schematic)

   Promoter

   Available since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNC1053

   Plasmid

   #20406

   Depositor

   Beverly Errede

   Article

   Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

   Insert

   CFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   linker: delta k

   Promoter

   Available since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNC829

   Plasmid

   #20386

   Depositor

   Beverly Errede

   Article

   Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

   Insert

   CFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNC860

    Plasmid

    #20390

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Linker: ek

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pINER3R

    Plasmid

    #185883

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIAeBlueHPV16LR

    Plasmid

    #185894

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIT7A6

    Plasmid

    #185896

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pINER4R

    Plasmid

    #185884

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRERF1-donor

    Plasmid

    #176366

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    TRERF1 (TRERF1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNR2E3-donor

    Plasmid

    #176369

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    NR2E3 (NR2E3 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRORB-donor

    Plasmid

    #176354

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    RORB (RORB Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZBED9-donor

    Plasmid

    #176344

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZBED9 (ZBED9 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTSC22D3-donor

    Plasmid

    #176355

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid

    #169359

    Purpose

    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    3XHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge