Addgene

We narrowed to 304 results for: FC2

Showing: 161 - 180 of 304 results

Results per page:

Sort results by:

CMVTO-KRAS

Plasmid

#179653

Purpose

KRAS protein

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-KRAS

Plasmid

#13919

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

KRAS 3'UTR (KRAS Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG2K

Plasmid

#35493

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

KRAS(G12V) (KRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Ubiquitin C

Available since

May 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
bactin:mKate2-ras

Plasmid

#105963

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Ras (KRAS Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-ER-KRAS(G12V)

Plasmid

#35635

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

ER

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0532

Plasmid

#143710

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PAX1 (PAX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-KRAS4A(G12V)

Plasmid

#35634

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4A(G12V) (KRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pCGN K-Ras 12V, 188L

Plasmid

#14722

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

K-ras (KRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12V, 188L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
TFORF0533

Plasmid

#143711

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PAX1 (PAX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Use

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FKBP:mCherry-KRas4Bc30

Plasmid

#72900

Purpose

Membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

GTPase K-Ras (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158..188 (fragment 1..157 deleted)

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
KRAS-P34R

Plasmid

#158527

Purpose

Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc1 K-RAS 4A

Plasmid

#236451

Purpose

transient overexpression of K-RAS 4A in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4A (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-MBP-KRas-Q61L

Plasmid

#214300

Purpose

Express constitutively active KRas fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active KRas (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-Q61L

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-MBP-KRas-S17N

Plasmid

#214301

Purpose

Express dominant negative KRas fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative KRas (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-S17N

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge