We narrowed to 446 results for: DHFR
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Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only
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GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHD22Y/PfPRMT5 deletion
Plasmid#203161Purposefor disruption of PfPRMT5 geneDepositorInsertPfPRMT5 fragment
Tags3 myc tags and 3' UTR of P. berghei dihydrof…ExpressionMammalianPromoterNoAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD22y/PfPRMT5-PTP
Plasmid#203160Purposefor tagging PTP to the 5' end of PfPRMT5 gene by single crossoverDepositorInsertPfPRMT5 fragment, PTP tag and pDT 3' UTR
Tags3' UTR of P. berghei dihydrofolate reductase…ExpressionMammalianPromoterNoAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL1360
Plasmid#219777PurposepDEF-TurboID-GFPmut2, empty vector for creating C-terminal fusion proteins.DepositorInsertTurboID/GFPmut2
UsePlasmodium, rodent malaria parasitesPromoterNoneAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1419
Plasmid#219778PurposepDEF-TurboID-GFPmut2, negative control for cytosolic expressionDepositorInsertTurboID/GFPmut2
UsePlasmodium, rodent malaria parasitesPromoterPbEF1alpha promoterAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL43C
Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
ExpressionYeastMutationDHFR (P66L), Rho1 (C17R)Available SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pERB260
Plasmid#67763PurposeExpresses BICD-mCherry-eDHFRDepositorInsertBICD-mCherry-eDHFR
TagsmCherryExpressionMammalianAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
ExpressionBacterialPromoterPdhfrAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98
Plasmid#130704PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-F98
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-F98 IRES EGFP
Plasmid#130710PurposePlasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-F98 IRES EGFP
TagsLyn11 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97
Plasmid#130705PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-G97
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
nSYB-GAL80-DD
Plasmid#124778PurposeExpresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoterDepositorInsertTMP-inducible destabilized GAL80 (GAL80-DD)
TagsDDExpressionInsectPromoternSynaptobrevinAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
3'-ecRESCUE-mCherry
Plasmid#191486PurposeecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherryDepositorUseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
10XUAS-flp-DD
Plasmid#124777PurposeExpresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized FLP (FLP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
TagsLyn11 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet
Plasmid#130708PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTP1
Plasmid#64952Purposesource of TpR FRT cassetteDepositorInsertFRT-dhfrII-FRT
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
10xUAS-GFP-DD
Plasmid#104989PurposeExpresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized GFP (GFP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES EGFP
Plasmid#130706PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet
Plasmid#130709PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-N95 (for GFP)
Plasmid#130716PurposePlasmid encoding the bacterial expression of LAMA-N95 for GFPDepositorInsertLAMA-N95
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPdhfrAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES mYFP
Plasmid#130712PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-F98-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES EGFP
Plasmid#130707PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES EGFP
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:DD-CreER; cryaa:mCerulean
Plasmid#230076PurposeExpression of destabilized CreER in zebrafish hepatocytes.DepositorInsertltDHFR(DD)
UseZebrafish expressionTagsCreER and HAMutationamino acids mutations: R12H, N23S, G67S, V78A, E1…Promoterfabp10aAvailable SinceMay 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DBJS-p8.35
Plasmid#246898PurposeUsed for multi-site piggybac transposon insertion of luciferase reporterDepositorInsertCNOT10::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
ExpressionMammalianAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
DBJS-p8.38
Plasmid#246899PurposeUsed for multi-site piggybac transposon insertion of luciferase reporterDepositorInsertDDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
ExpressionMammalianAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
DBJS-p8.32
Plasmid#246897PurposeUsed for multi-site piggybac transposon insertion of luciferase reporterDepositorInsertRB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
ExpressionMammalianAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-S98 (for p24)
Plasmid#130717PurposePlasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid proteinDepositorInsertEGFP-LAMA-S98
TagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG1-COMP5AP-AviTag-9xHis
Plasmid#157379PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG1-Fc(DAPA)-AviTag-6xHis
Plasmid#156815PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertPSG1 (PSG1 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only