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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 443 results for: DHFR

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Showing: 401 - 443 of 443 results

  1. pIRHDGAL43C

    Plasmid
    #218279
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218279-plasmid-map-sequence-id-433681

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  2. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218276-plasmid-map-sequence-id-433677

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  10. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid
    #117911
    Purpose
    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM
    Depositor
    Kai Johnsson
    Article
    Yu et al Science. 2018 Sep 14;361(6407):1122-1126
    Insert
    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    R44A & H45Q on eDHFR
    Promoter
    T7
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    117911-plasmid-map-sequence-id-226235

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  12. DBJS-p8.35

    Plasmid
    #246898
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    CNOT10::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246898-plasmid-map-sequence-id-490586

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  13. DBJS-p8.38

    Plasmid
    #246899
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    DDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246899-plasmid-map-sequence-id-490573

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  14. DBJS-p8.32

    Plasmid
    #246897
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    RB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246897-plasmid-map-sequence-id-490570

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  36. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  37. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  38. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  41. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

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Showing: 401 - 443 of 443 results