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pRDAX-EMMI-018 IRES-A[G338A](H-I)//Esp3I

Plasmid

#130220

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-A[G338A]

Use

Synthetic Biology

Mutation

G338A

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

Plasmid

#61935

Purpose

Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, residues Q337 an…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pNP3386 [For use in genome insertion and off-target assessment]

Plasmid

#247258

Purpose

Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 D337, 338, 339A

Plasmid

#38280

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed D337A, D338A, D339A

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
AiP1099 - pAAV-AiE0338m-minBG-FlpO-WPRE-HGHpA

Plasmid

#230570

Purpose

AiE0338m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO

Use

AAV

Promoter

minBG

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRDAX-338 N-Tag UnaG[1] L10 {Esp3I(2x)} EBPF2

Plasmid

#129841

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]

Plasmid

#107852

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348V in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)

Plasmid

#110144

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Tags

cogGFP

Expression

Bacterial and Plant

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEM112C

Plasmid

#250587

Purpose

H2Apr-Lifeact-mClover3-H2Ater

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-Lifeact-mClover3-H2Ater

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Fp16SrRNA

Plasmid

#218519

Purpose

pUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Bv16SrRNA

Plasmid

#218518

Purpose

pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEM110

Plasmid

#250586

Purpose

H2Apr-MRLC-mClover3-FLAG-MRLCter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-SpunMLRC-mClover3-SpunMLRCter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pEM102

Plasmid

#250585

Purpose

AlphaTUBpr-mClover3-BdenAlphaTUB-BdTUBter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

SpunaTubpr-mClover3-Bden-aTub-BdenaTubter:H2Bpr-P2A-T2A-HygR-Synther8

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-eGFP-NMHC-IIAdeltaIQ2

Plasmid

#35690

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP and GFPGenotRW

Expression

Mammalian

Mutation

The coding segment for aa 804-834 (corresponding …

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
DN-LOXL2

Plasmid

#200062

Purpose

LOXL2 deletion mutant lacking 1-338 amino acids

Depositor

Lakshmi Santhanam

Article

Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

Insert

human lysyl oxidase like 2 (LOXL2 Human)

Tags

His

Expression

Mammalian

Mutation

N-terminus amino acids 1-338 truncated.

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

Plasmid

#138334

Purpose

Expresses APOB-Linker-eGFP

Depositor

Mahmood Hussain

Article

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Insert

5'UTR-ApoB48-Linker-eGFP (APOB Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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