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Plasmid#179334PurposeNT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).DepositorInsertPtac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationCas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationPromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AP1297‐1
Plasmid#87247Purposegtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
UseTagsgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…ExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-TNKS-2
Plasmid#34691DepositorInsertTNKS2 (TNKS2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-TNKS-2-M1054V
Plasmid#34692DepositorInsertTNKS2-M1054V (TNKS2 Human)
UseTagsFLAGExpressionMammalianMutationM1054VPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iCreT2ALuc-L3 #BGV208
Plasmid#48992PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertscre recombinase
Luciferase
UseLuciferase; Gateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterUnknownAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-dsRed-L3 #BGV203
Plasmid#48973PurposeSupplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertdsRed
UseGateway cloning vectorTagsIRES (strong)ExpressionMutationPromoternoneAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iBlast-L3 #BGV204
Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iHygro-L3 #BGV206
Plasmid#48985PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserthygromycin phosphotransferase
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase
Plasmid#188391PurposeReporter for BCAM expressionDepositorInsertBCAM reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iPuro-L3 #BGV205
Plasmid#48987PurposeSupplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertpuromycin resistance
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eGFP-L3 #BGV201
Plasmid#48979PurposeSupplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteGFP
UseGateway cloning vectorTagsIRES (strong)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iNeo-L3 #BGV207
Plasmid#48986PurposeSupplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertneomycin resistance
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eCFP-L3 #BGV202
Plasmid#48977PurposeSupplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteCFP
UseGateway cloning vectorTagsIRES (strong)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-F1
Plasmid#194592PurposeBacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-E2
Plasmid#194590PurposeBacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertE2 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-G6
Plasmid#194589PurposeBacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-C7
Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only