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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 557 results for: BIG;

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Showing: 161 - 180 of 557 results

  2. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221772-plasmid-map-sequence-id-455976

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  4. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

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  7. pD649-HAsp-CNTN4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157017
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CNTN4 (CNTN4 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157017-plasmid-map-sequence-id-303143

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Showing: 161 - 180 of 557 results