Addgene

We narrowed to 4,075 results for: PRS

Showing: 161 - 180 of 4075 results

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pRSF_ApyA

Plasmid

#226249

Purpose

Production of unmodified ApyA peptide.

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Insert

His6_ApyA

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyADO

Plasmid

#226248

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAO

Plasmid

#226242

Purpose

Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAD

Plasmid

#226241

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET.HaloTag.v7

Plasmid

#226197

Purpose

bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Insert

HaloTag.v7

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ERG

Plasmid

#205943

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ERG (TMPRSS2, ERG Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ETV1

Plasmid

#205944

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ETV1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ETV1 (TMPRSS2, ETV1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Shim_TrpRS_TP_GFP_pK7FWG2

Plasmid

#202653

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic TrpRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_AspRS_TP_GFP_pK7FWG2

Plasmid

#202647

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic AspRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRS193

Plasmid

#197143

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS138

Plasmid

#197142

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETA_crG18

Plasmid

#172930

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

Depositor

Arnold Boersma

Article

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorG18

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRS_1N_400

Plasmid

#170476

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

Insert

T7A1, O1, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS_2S_400

Plasmid

#170478

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, O2, Os, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS_2N_400

Plasmid

#170479

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, O2, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3G919C

Plasmid

#120255

Purpose

Expression of ATP3-7 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G919 changed to C; Alanine 307 changed to Proline

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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