We narrowed to 505 results for: SPY

1. pHR-SIN-BX SpyCatcher003-hCD52

   Plasmid

   #210565

   Purpose

   Expresses Spycatcher003 on the cell surface using human CD52 hinge

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to human CD52 hinge
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b 6His SpyCatcher-GRFT

   Plasmid

   #204502

   Purpose

   Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

   Depositor

   Urartu Seker

   Article

   Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

   Insert

   GRFT
   

   Use

   Synthetic Biology

   Tags

   6xHis and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpyCas9 EMX1-nicking sgRNA

   Plasmid

   #169859

   Purpose

   SpyCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpyCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXGFPC-5 Pxyl-dcas9 (Spy)

   Plasmid

   #133316

   Purpose

   expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-sfGFP-SpyTag003 Loop A

   Plasmid

   #171776

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues V…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFN-HaloTag-TalinR8-SpyTag

   Plasmid

   #192045

   Purpose

   Plasmid expresses human talin R8 domain flanked between a HaloTag and a SpyTag

   Depositor

   Ionel Popa

   Article

   Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

   Insert

   TLN1 (TLN1 Human)
   

   Use

   Tags

   HaloTag and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-P3-IgKL-SpyCatcher003-GINKO2

   Plasmid

   #234544

   Purpose

   to express a potassium sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-GINKO2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

   Plasmid

   #234543

   Purpose

   to express a green fluorescent protein tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-P3-IgKL-SpyCatcher003-SEP

   Plasmid

   #234541

   Purpose

   to express a pH sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-SEP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dSpyCas9-E566-GS-LOV2-VPR

    Plasmid

    #231576

    Purpose

    Potent light-deactivated CRISPRa

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    dSpyCas9-VPR with an AsLOV2 insertion behind E566
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-Spy-CD28(CD45TM)

    Plasmid

    #223624

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-Spy-CD28(Y191F)

    Plasmid

    #223623

    Purpose

    Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    Y191F

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid

    #216307

    Purpose

    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

    Plasmid

    #216281

    Purpose

    Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-SpyTag-(AffiHER2)x3
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyTag-RBD Omicron BQ.1.1

    Plasmid

    #214725

    Purpose

    Expresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

    Insert

    Receptor-Binding Domain (S SARS-CoV-2)
    

    Use

    Tags

    SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST14-Cys-SpyCatcher (Min)

    Plasmid

    #216713

    Purpose

    Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

    Depositor

    Alex Dunn

    Article

    Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

    Insert

    Cys-SpyCatcher (Min)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See Li...Howarth, J. Mol. Biol., 2014

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZa pLtetO CsgA-SpyTag

    Plasmid

    #204503

    Purpose

    Bacterial expression of CsgA with C-terminal SpyTag peptide and GS linker

    Depositor

    Urartu Seker

    Article

    Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

    Insert

    CsgA
    

    Use

    Synthetic Biology

    Tags

    6xHis, SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFN-HaloTag-TalinR7R8-SpyTag

    Plasmid

    #192046

    Purpose

    Plasmid expresses human talin R7R8 domain flanked between a HaloTag and a SpyTag

    Depositor

    Ionel Popa

    Article

    Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

    Insert

    TLN1 (TLN1 Human)
    

    Use

    Tags

    HaloTag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge