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We narrowed to 705 results for: intein

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Showing: 161 - 180 of 705 results

  9. phTERT-cp PS Intein tTA

    Plasmid
    #242032
    Purpose
    Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cp PS Intein tTA
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242032-plasmid-map-sequence-id-483130

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  10. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Publication
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  11. pNF-KB-Split PS Intein-C tTA

    Plasmid
    #242035
    Purpose
    C-terminal segment of a blue-light-controlled split PS Intein tTA gene expression system, co-expressing mCherry under an NF-KB-inducible promoter
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-C tTA
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The CMV promoter in the original vector was repla…
    Promoter
    NF-KB
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242035-plasmid-map-sequence-id-483142

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  16. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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Showing: 161 - 180 of 705 results